Ankyrin 3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-05549

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Monkey

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Format

BSA Free
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Product Specifications

Immunogen

The C-terminal 398 amino acids of human Ankyrin 3 expressed in and purified from E. coli.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Scientific Data Images for Ankyrin 3 Antibody - BSA Free

Western Blot: Ankyrin 3 Antibody [NBP3-05549]

Western Blot: Ankyrin 3 Antibody [NBP3-05549]

Western Blot: Ankyrin 3 Antibody [NBP3-05549] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody at a dilution of 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction. Bands at 60-190kDa represent Ankyrin 3 splice variants and proteolytic fragments. Larger Ankyrin 3 isoforms of 270kDa and 480kDa can be seen on longer exposure of the blots.
Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody [NBP3-05549]

Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody [NBP3-05549]

Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05549] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Ankyrin 3 Antibody, CPCA-ANK3, at a dilution of 1:2,000 in green, and costained with mouse mAb to RBFOX3/NeuN at a dilution of 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The Ankyrin 3 antibody stains the axonal initial segments, while the RBFOX3/NeuN antibody reveals perikarya and nuclei of neurons.

Applications for Ankyrin 3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Immunohistochemistry

1:500-1:1000

Western Blot

1:5000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol. Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.

Format

BSA Free

Preservative

5mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: Ankyrin 3

Alternate Names

ankyrin G, Ankyrin G119, ankyrin-3, ankyrin-G, Node Of Ranvier

Gene Symbol

ANK3

Additional Ankyrin 3 Products

Product Documents for Ankyrin 3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Ankyrin 3 Antibody - BSA Free

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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