Ankyrin Brain Antibody (S105-13) - BSA Free
Novus Biologicals | Catalog # NBP2-59374
![Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374]](https://resources.rndsystems.com/images/products/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # S105-13
Format
BSA Free
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Product Specifications
Immunogen
Synthetic peptide amino acids 203-496 of human Ankyrin-B
Localization
Cytoplasm, Cytoskeleton, Myofibril, Sarcomere, M line, Apical Cell Membrane, Cell Membrane
Specificity
Detects 200kDa. No cross reactivity against Ankyrin-G.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Ankyrin Brain Antibody (S105-13) - BSA Free
Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374]
Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin Brain Antibody (D) Composite.![Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374]](https://resources.rndsystems.com/images/products/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374]")
Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374]
Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm![Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374]](https://resources.rndsystems.com/images/products/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374]")
Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374]
Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: CytoplasmApplications for Ankyrin Brain Antibody (S105-13) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Immunohistochemistry
1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Ankyrin Brain
Alternate Names
Ank2, Ankyrin 2, Ankyrin 2 brain, Ankyrin B, Ankyrin brain, Ankyrin nonerythroid, Brain Ankyrin
Gene Symbol
ANK2
Additional Ankyrin Brain Products
Product Documents for Ankyrin Brain Antibody (S105-13) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Ankyrin Brain Antibody (S105-13) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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