Annexin A2 Antibody (10H9) - BSA Free

Novus Biologicals | Catalog # NBP3-26506

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10H9 expressed in HEK293

Format

BSA Free
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Product Specifications

Immunogen

A synthesized peptide derived from Human Annexin A2 [UniProt P07355]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Annexin A2 Antibody (10H9) - BSA Free (NBP3-26506) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Annexin A2 Antibody (10H9) - BSA Free

Annexin A2 Antibody (10H9)

Immunohistochemistry: Annexin A2 Antibody (10H9) [NBP3-26506] -

Immunohistochemistry: Annexin A2 Antibody (10H9) [NBP3-26506] - Image of Annexin A2 Antibody (10H9) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Annexin A2 Antibody (10H9)

Western Blot: Annexin A2 Antibody (10H9) [NBP3-26506] -

Western Blot: Annexin A2 Antibody (10H9) [NBP3-26506] - Positive Western Blot detected in: HEK293 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate.
All lanes: Annexin A2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 39, 41 kDa
Observed band size: 36-45 kDa

Applications for Annexin A2 Antibody (10H9) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Annexin A2

The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin II is a calcium regulated, membrane binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ANX2L4, ANXA2, CAL1H, LIP2, Lipocortin-2, LPC2D, PAP-IV

Gene Symbol

ANXA2

Additional Annexin A2 Products

Product Documents for Annexin A2 Antibody (10H9) - BSA Free

Certificate of Analysis

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Product Specific Notices for Annexin A2 Antibody (10H9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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