Antidiuretic Hormone Antibody - BSA Free

Novus Biologicals | Catalog # NB100-65214

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, ELISA

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Antidiuretic Hormone Primary Antibodies »

Product Specifications

Immunogen

Synthetic Antidiuretic Hormone conjugated to bovine thyroglobulin

Reactivity Notes

Broad species cross-reactivity expected from sequence.

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Hypothalamus and posterior lobe of the pituitary gland are reliable positive control tissues for IHC experiments.

Applications for Antidiuretic Hormone Antibody - BSA Free

Application
Recommended Usage

ELISA

1:2000 - 1:20000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

5.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Antidiuretic Hormone

This gene encodes a member of the vasopressin/oxytocin family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the neuropeptide hormone arginine vasopressin, and two other peptides, neurophysin 2 and copeptin. Arginine vasopressin is a posterior pituitary hormone that is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). This gene is present in a gene cluster with the related gene oxytocin on chromosome 20. [provided by RefSeq, Nov 2015]

Alternate Names

ADH, arginine vasopressin, ARVP, Vasopressin

Entrez Gene IDs

551 (Human); 11998 (Mouse); 24221 (Rat); 280728 (Bovine)

Gene Symbol

AVP

UniProt

P01185, PO1185 (Human)

Additional Antidiuretic Hormone Products

Product Documents for Antidiuretic Hormone Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Antidiuretic Hormone Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Antidiuretic Hormone Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Antidiuretic Hormone Antibody - BSA Free

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  • Q: We ordered the Arg-vasopressin Antibody (NB100-65214) (0.05 ml) last week and also received it. I was wondering whether you have any idea about the working dilution since there is nothing indicated in the datasheet?

    A: The recommended starting dilution for IHC is 1:100-1:200 and for IF we recommend 1:50-1:250. These are just starting dilutions and should be optimized.

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