![Western Blot: AP3D1 Antibody [NBP2-27158]](https://resources.rndsystems.com/images/products/AP3D1-Antibody-Western-Blot-NBP2-27158-img0001.jpg "Western Blot: AP3D1 Antibody [NBP2-27158]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Primate (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
A portion of amino acids 530-580 of human AP3 Delta was used as the immunogen for the antibody.
Reactivity Notes
The AP3 amino acid sequence used as immunogen for this antibody is 100% homologous in human, chimpanzee, orangutan, new world monkey, rhesus monkey, and 63% homologous in panda, carolina anole, opossum, and 58% homologous in taipan, zebra finch, horse, dog, pig and 53% homologous in cow, rat, mouse.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AP3D1 Antibody
Western Blot: AP3D1 Antibody [NBP2-27158]
Western Blot: AP3D1 Antibody [NBP2-27158] - analysis using AP3 Delta antibody. HeLa lysate in the 1) absence and 2) presence of immunizing peptide, probed with AP3 Delta antibody at 2 ug/ml. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.![Immunohistochemistry-Paraffin: AP3D1 Antibody [NBP2-27158]](https://resources.rndsystems.com/images/products/AP3D1-Antibody-Immunohistochemistry-Paraffin-NBP2-27158-img0002.jpg "Immunohistochemistry-Paraffin: AP3D1 Antibody [NBP2-27158]")
Immunohistochemistry-Paraffin: AP3D1 Antibody [NBP2-27158]
Immunohistochemistry-Paraffin: AP3D1 Antibody [NBP2-27158] - IHC of human rectum tissue probed with AP3 Delta antibody at 5 ug/ml.Applications for AP3D1 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
5ug/ml
Western Blot
2-5ug/ml~
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AP3D1
Alternate Names
Adapter-related protein complex 3 subunit delta-1, adaptin, delta, adaptor-related protein complex 3, delta 1 subunit, ADTD, AP-3 complex delta subunit, partial CDS, AP-3 complex subunit delta, AP-3 complex subunit delta-1, delta adaptin, Delta-adaptin, delta-adaptin, partial CDS, hBLVR, subunit of putative vesicle coat adaptor complex AP-3
Gene Symbol
AP3D1
Additional AP3D1 Products
Product Documents for AP3D1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AP3D1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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