ApoE4 Antibody (4E4) - BSA Free

Novus Biologicals | Catalog # NBP1-49529

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4E4

Format

BSA Free
View all ApoE4 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal region of human ApoE4 (within residues 100-150) [Uniprot: P02649]

Localization

Secreted

Specificity

This antibody is specific for ApoE4. It does not cross-react with the ApoE2 or ApoE3 isoforms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ApoE4 Antibody (4E4) - BSA Free

Western Blot: ApoE4 Antibody (4E4)BSA Free [NBP1-49529]

Western Blot: ApoE4 Antibody (4E4)BSA Free [NBP1-49529]

Western Blot: ApoE4 Antibody (4E4) [NBP1-49529] - Analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using NBP1-49529. Image courtesy of Dr. Marko Roblek from the Medical University of Vienna.
Immunocytochemistry/ Immunofluorescence: ApoE4 Antibody (4E4) - BSA Free [NBP1-49529]

Immunocytochemistry/ Immunofluorescence: ApoE4 Antibody (4E4) - BSA Free [NBP1-49529]

Immunocytochemistry/Immunofluorescence: ApoE4 Antibody (4E4) [NBP1-49529] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ApoE4 (4E4) at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Applications for ApoE4 Antibody (4E4) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2-5 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1:1000-1:2000
Application Notes
In Western blot, a band can been seen at approximately 34 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ApoE4

Apolipoprotein E has three isoforms namely ApoE2, ApoE3, and ApoE4 that differ from each other by single amino acid interchanges at residues 130/176, and ApoE4 is an isoforms which contains arginine at 130 and 176 locations. ApoE4 is mainly synthesized in liver and brain, and is widely expressed in other tissues also. ApoE4 functions have been linked to neuronal plasticity, synaptogenesis etc. and ApoE4 expressing subjects have reduced capacity for synaptic plasticity, increased amyloid-beta deposition, and early onset of Alzheimer's disease (AD). ApoE4 is also linked to progression/poor clinical outcomes in TBI (traumatic brain injury), multiple sclerosis, stroke, frontotemporal dementia, and Parkinson's disease. The neuropathological effects of apoE4 includes impaired neurite outgrowth, cytoskeletal disruption/hyperphosphorylation of tau, neuronal mitochondrial dysfunction, impaired synaptogenesis, increased amyloid beta production as well as clearance/deposition, increased lysosomal leakage and neuronal apoptosis, cerebral neuropathology and impaired learning and memory.

Long Name

Apolipoprotein E4

Alternate Names

Apo-E, apolipoprotein E, apolipoprotein E3, late onset), LDLCQ5, MGC1571

Entrez Gene IDs

348 (Human)

Gene Symbol

APOE

UniProt

P02649

Additional ApoE4 Products

Product Documents for ApoE4 Antibody (4E4) - BSA Free

Certificate of Analysis

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Product Specific Notices for ApoE4 Antibody (4E4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ApoE4 Antibody (4E4) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ApoE4 Antibody (4E4) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Hello! I was wondering if NBP1-49529 ApoE4 Antibody (4E4) detects any other isoform of ApoE

    A: This antibody is specific for ApoE4. It does not cross-react with the ApoE2 or ApoE3 isoforms. Please see the WB image.

  • Q: I'm interested in your product ApoE4 Ab (NBP1-49529). According to your web information, it is specific to ApoE4 only, no cross interaction with E2 and E3. Is that right? Would you please let me know if your have any references for this? Has anyone tried in their experiments? How was it?

    A: Unfortunately I do not have any references on file regarding this antibody that I can pass along at this time. The lab has indicated that it will not cross react and therefore we will stand by that claim with our 100% guarantee. That way if you do see any cross reaction we would be happy to send you a replacement or refund. The western blot image on the datasheet should also help answer your question.

  • Q: Hello! I was wondering if NBP1-49529 ApoE4 Antibody (4E4) detects any other isoform of ApoE

    A: This antibody is specific for ApoE4. It does not cross-react with the ApoE2 or ApoE3 isoforms. Please see the WB image.

  • Q: I'm interested in your product ApoE4 Ab (NBP1-49529). According to your web information, it is specific to ApoE4 only, no cross interaction with E2 and E3. Is that right? Would you please let me know if your have any references for this? Has anyone tried in their experiments? How was it?

    A: Unfortunately I do not have any references on file regarding this antibody that I can pass along at this time. The lab has indicated that it will not cross react and therefore we will stand by that claim with our 100% guarantee. That way if you do see any cross reaction we would be happy to send you a replacement or refund. The western blot image on the datasheet should also help answer your question.

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