Apolipoprotein M/ApoM Antibody

Novus Biologicals | Catalog # NB100-57088

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence PRNQEACELSNN corresponding to C-Terminus according to NP_061974.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Apolipoprotein M/ApoM Antibody

Immunohistochemistry-Paraffin: Apolipoprotein M/ApoM Antibody [NB100-57088]

Immunohistochemistry-Paraffin: Apolipoprotein M/ApoM Antibody [NB100-57088]

Immunohistochemistry-Paraffin: Apolipoprotein M/ApoM Antibody [NB100-57088] - (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Apolipoprotein M/ApoM Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

3 - 6 ug/ml

Peptide ELISA

Detection limit 1:8000
Application Notes
WB: Preliminary experiments gave an approx. 50 kDa band in human kidney and liver lysates after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.3 kDa band according to NP_061974.2. We do note that ApoM is a glycoprotein which makes a higher size band to be expected. The 50 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human liver shows strong stain of cytoplasm in select hepatocytes.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Apolipoprotein M/ApoM

The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Two transcript variants encoding two different isoforms have been found for this gene, but only one of them has been fully characterized.

Alternate Names

APOM, NG20, Protein G3a

Entrez Gene IDs

55937 (Human)

Gene Symbol

APOM

UniProt

Additional Apolipoprotein M/ApoM Products

Product Documents for Apolipoprotein M/ApoM Antibody

Certificate of Analysis

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Product Specific Notices for Apolipoprotein M/ApoM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for Apolipoprotein M/ApoM Antibody

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Protocols

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