APP Antibody - Azide Free

Novus Biologicals | Catalog # NB300-308

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Rat

Predicted:

Canine (100%), Chicken (100%), Human (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all APP Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # P08592

Reactivity Notes

It is anticipated that the antibody will also work with canine, chicken, human, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Localization

Cell Membrane, Cytoplasmic, Endoplasmic reticulum and Golgi Apparatus

Specificity

Specific for endogenous levels of the ~115 kDa APP protein. Immunolabeling of the APP protein band is completely blocked by preadsorption of the antibody with the peptide used as antigen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit APP Antibody - Azide Free (NB300-308) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-APP Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for APP Antibody - Azide Free

Western Blot: APP Antibody [NB300-308]

Western Blot: APP Antibody [NB300-308]

Western Blot: APP Antibody [NB300-308]
Immunocytochemistry/ Immunofluorescence: APP Antibody [NB300-308]

Immunocytochemistry/ Immunofluorescence: APP Antibody [NB300-308]

APP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-308-img0002.jpg
APP Antibody

Western Blot: APP Antibody [NB300-308] -

Western Blot: APP Antibody [NB300-308] - Cell surface staining of VDR (green) on live neurons followed by fixation & immunofluorescence labeling of target proteins (100x): A) ADAM10; B) APP; C) BACE1; D) Nicastrin; E) Presenilin 1; F) Presenilin 2. Overlay images indicate the possible co-localization of VDR/ADAM10 (A) or VDR/APP (B) or VDR/Nicastrin (D) or limited co-localization of VDR/ Presenilin 1 (E) on the neuronal plasma membrane. Overlay images do not indicate co-localization for VDR/BACE1 (C) or VDR/Presenilin 2 (F) on the neuronal plasma membrane. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29176823), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for APP Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
Immunolabeling of the APP protein band is completely blocked by preadsorption of the with the peptide used as antigen. Expected band seen at ~115 kDa in Western blots. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID : 29176823).

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: APP

A large body of evidence has implicated the amyloid precursor protein (APP) in the pathogenesis of Alzheimer's disease (AD). The phosphorylation APP at Thr668 is thought to play a critical role in generation of the soluble APP (beta) and beta-amyloid peptide (abeta) which are the major components of senile plaques in patient brains inflicted with AD.

Long Name

Amyloid Precursor Protein

Alternate Names

Amyloid beta, beta Amyloid, Protease Nexin II

Entrez Gene IDs

351 (Human); 107417 (Mouse); 29722 (Rat)

Gene Symbol

APP

UniProt

P08592 (Rat)

Additional APP Products

Product Documents for APP Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for APP Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for APP Antibody - Azide Free

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Protocols

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