Aquaporin 1/AQP1 Antibody (JM10-98)

Novus Biologicals | Catalog # NBP2-67162

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM10-98 expressed in HEK293
View all Aquaporin 1/AQP1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Aquaporin 1/AQP1 aa 245-269 / 269. (SwissProt: P29972 Human; SwissProt: Q02013 Mouse; SwissProt: P29975 Rat)

Localization

Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aquaporin 1/AQP1 Antibody (JM10-98)

Western Blot: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Western Blot: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Western Blot: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162] - Analysis of AQP1 on different cells lysates using anti-AQP1 antibody at 1/500 dilution. Positive control: Line 1: Hela Line 2:Jurkat Line 3: Human kidney
Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunocytochemistry/Immunofluorescence: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162] - Staining AQP1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-AQP1 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunocytochemistry/Immunofluorescence: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162] - Staining AQP1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunocytochemistry/Immunofluorescence: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162] - Staining AQP1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162] - Analysis of paraffin-embedded human breast tissue using anti-AQP1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162] - Analysis of paraffin-embedded human kidney tissue using anti-AQP1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162] - Analysis of paraffin-embedded human pancreas tissue using anti-AQP1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162] - Analysis of paraffin-embedded human spleen tissue using anti-AQP1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162] - Analysis of paraffin-embedded mouse colon tissue using anti-AQP1 antibody. Counter stained with hematoxylin.
Aquaporin 1/AQP1 Antibody (JM10-98) Western Blot: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Western Blot: Aquaporin 1/AQP1 Antibody (JM10-98) [NBP2-67162]

Western blot analysis on different lysates with NBP2-67162 at 1/5,000 dilution. Lane 1: Mouse kidney tissue lysate Lane 2: Mouse kidney tissue lysate (no heat) Lane 3: Mouse lung tissue lysate Lane 4: Mouse lung tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 28 kDa Observed band size: 28~40 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67162 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for Aquaporin 1/AQP1 Antibody (JM10-98)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:800

Multiplex Immunofluorescence

1:5000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aquaporin 1/AQP1

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Thisgene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayerspanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant inerythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involvingimbalance in ocular fluid movement. (provided by RefSeq)

Alternate Names

AQP1, CHIP28, CO

Gene Symbol

AQP1

Additional Aquaporin 1/AQP1 Products

Product Documents for Aquaporin 1/AQP1 Antibody (JM10-98)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Aquaporin 1/AQP1 Antibody (JM10-98)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Ion Channels and Regulators

Citations for Aquaporin 1/AQP1 Antibody (JM10-98)

Customer Reviews for Aquaporin 1/AQP1 Antibody (JM10-98)

There are currently no reviews for this product. Be the first to review Aquaporin 1/AQP1 Antibody (JM10-98) and earn rewards!

Have you used Aquaporin 1/AQP1 Antibody (JM10-98)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Aquaporin 1/AQP1 Antibody (JM10-98)

Showing  1 - 1 of 1 FAQ Showing All

  • Q: Are any of your Aquaporin-1 antibodies suitable for live cell staining? I am looking at doing flow cytometry on live cells and further isolation of the positive cells to purify the population.

    A:

    A list of our Aquaporin-1 antibodies suitable for use in flow cytometry can be found using this link. Of note, NB600-741 is known to bind to an extracellular domain, so should be suitable for your use.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies