Aquaporin-4 Antibody (CL0178)
Novus Biologicals | Catalog # NBP3-52095
Recombinant Monoclonal Antibody
![Immunohistochemistry: Aquaporin-4 Antibody (CL0178) [NBP3-52095]](https://resources.rndsystems.com/images/products/nbp3-52095_mouse-aquaporin-4-mab-cl0178-immunohistochemistry-273202615284419.jpg "Immunohistochemistry: Aquaporin-4 Antibody (CL0178) [NBP3-52095]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # CL0178
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P55087, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Aquaporin-4 Antibody (CL0178)
Immunohistochemistry: Aquaporin-4 Antibody (CL0178) [NBP3-52095]
Staining of human stomach shows moderate to strong membranous positivity in glandular cells.![Immunohistochemistry: Aquaporin-4 Antibody (CL0178) [NBP3-52095]](https://resources.rndsystems.com/images/products/nbp3-52095_mouse-aquaporin-4-mab-cl0178-immunohistochemistry-27320261519617.jpg "Immunohistochemistry: Aquaporin-4 Antibody (CL0178) [NBP3-52095]")
Immunohistochemistry: Aquaporin-4 Antibody (CL0178) [NBP3-52095]
Staining of human cerebral cortex shows moderate to strong membranous positivity in astrocytes.![Immunohistochemistry: Aquaporin-4 Antibody (CL0178) [NBP3-52095]](https://resources.rndsystems.com/images/products/nbp3-52095_mouse-aquaporin-4-mab-cl0178-immunohistochemistry-273202615202423.jpg "Immunohistochemistry: Aquaporin-4 Antibody (CL0178) [NBP3-52095]")
Immunohistochemistry: Aquaporin-4 Antibody (CL0178) [NBP3-52095]
Analysis in human cerebral cortex and liver tissues. Corresponding Aquaporin-4 RNA-seq data are presented for the same tissues.![Immunohistochemistry: Aquaporin-4 Antibody (CL0178) [NBP3-52095]](https://resources.rndsystems.com/images/products/nbp3-52095_mouse-aquaporin-4-mab-cl0178-immunohistochemistry-2732026151965.jpg "Immunohistochemistry: Aquaporin-4 Antibody (CL0178) [NBP3-52095]")
Immunohistochemistry: Aquaporin-4 Antibody (CL0178) [NBP3-52095]
Staining of human cerebellum shows strong positivity in neuropil, mainly in the molecular layer.![Western Blot: Aquaporin-4 Antibody (CL0178) [NBP3-52095]](https://resources.rndsystems.com/images/products/nbp3-52095_mouse-aquaporin-4-mab-cl0178-western-blot-2732026152699.jpg "Western Blot: Aquaporin-4 Antibody (CL0178) [NBP3-52095]")
Western Blot: Aquaporin-4 Antibody (CL0178) [NBP3-52095]
Analysis in human cerebral cortex tissue.![Immunohistochemistry: Aquaporin-4 Antibody (CL0178) [NBP3-52095]](https://resources.rndsystems.com/images/products/nbp3-52095_mouse-aquaporin-4-mab-cl0178-immunohistochemistry-273202615275519.jpg "Immunohistochemistry: Aquaporin-4 Antibody (CL0178) [NBP3-52095]")
Immunohistochemistry: Aquaporin-4 Antibody (CL0178) [NBP3-52095]
Staining of human liver shows no positivity in hepatocytes as expected.Applications for Aquaporin-4 Antibody (CL0178)
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aquaporin 4/AQP4
Long Name
Aquaporin 4
Alternate Names
AQP-4, AQP4, MIWC, WCH4
Gene Symbol
AQP4
Additional Aquaporin 4/AQP4 Products
Product Documents for Aquaporin-4 Antibody (CL0178)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Aquaporin-4 Antibody (CL0178)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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