Aquaporin-4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-97928

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Aquaporin 4/AQP4 Primary Antibodies »

Product Specifications

Immunogen

Produced against the C-terminal peptide (Sequence N-CTKGSYMEVEDNRSQVETED) of rat aquaporin 4

Localization

Membrane

Marker

Astrocytes Marker

Specificity

Detects approx 35kDa. May detect larger glycosylated bands approx 50kDa.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aquaporin-4 Antibody - BSA Free

Western Blot: Aquaporin-4 Antibody [NBP1-97928]

Western Blot: Aquaporin-4 Antibody [NBP1-97928]

Western Blot: Aquaporin-4 Antibody [NBP1-97928] - Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin-4 protein using Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928). Primary Antibody: Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928) at 1:2000.
Immunohistochemistry: Aquaporin-4 Antibody [NBP1-97928]

Immunohistochemistry: Aquaporin-4 Antibody [NBP1-97928]

Immunohistochemistry: Aquaporin-4 Antibody [NBP1-97928] - Immunohistochemistry analysis using Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin-4 Polyclonal Antibody (NBP1-97928) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 m cryostat sections.

Applications for Aquaporin-4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:400

Immunohistochemistry

1:200

Western Blot

1:2000
Application Notes
0.5 ug/ml of Aquaporin 4 Antibody was sufficient for detection of aquaporin 4 in 10 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aquaporin 4/AQP4

The Aquaporin-4 gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selectivechannels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Twoalternatively spliced tra

Long Name

Aquaporin 4

Alternate Names

AQP-4, AQP4, MIWC, WCH4

Gene Symbol

AQP4

UniProt

NP_036957.1 (Rat)

Additional Aquaporin 4/AQP4 Products

Product Documents for Aquaporin-4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Aquaporin-4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Ion Channels and Regulators

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Protocols

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