AQUApure Tri-Ub Chains (K11-linked) Protein, CF

Catalog # Availability Size / Price Qty
UC-50-025
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AQUApure Tri-Ub Chains (K11-linked) Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

 

Ub-AQUA analysis:

K11:   99.14%

K63:     0.52%

K6  :     0.15%

K33:     0.12%

All other linkages ≤ 0.03%

Activity

Ubiquitin chains vary in length, linkage, and function. K11-linked Tri-Ubiquitin Chains (Ub3) are ideal for investigating Ubiquitin-binding proteins and as substrates for Ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40 °C for 20 minutes prior to gel electrophoresis.

Source
E. coli-derived human Tri-Ubiquitin protein
Accession #
Predicted Molecular Mass
26 kDa

Product Datasheets

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Background: Tri-Ubiquitin

Linkage specific Poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB's") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules. Poly-Ubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBAs), Ubiquitin-interacting motifs (UIMs), ZnF's and/or other Ubiquitin-sensing elements.

K11-linked Tri-Ubiquitin chains are manufactured using recombinant Ubiquitin and purely enzymatic techniques to avoid the potential for contaminating synthetic intermediates. The correctness of linkage and purity of each production lot is assessed using the Absolute Quantitation of Ubiquitin method (Ub-AQUA), an LCMS-based technique that provides extremely accurate information on the composition of Poly-Ubiquitin samples.

References
  1. Kirkpatrick D.S., et al. (2006) Nat Cell Biol.  8(7): 700-10
  2. Ordureau, A., et al. (2014) Mol. Cell  56(3): 360–375
  3. Ordureau, A., et al. (2015) Pro. Nat. Acad. of Sci. USA  112(21): 6637–6642
  4. Phu L., et al. (2011) Mol Cell Proteomics  10(5): M110.003756
Entrez Gene IDs
7314 (Human)
Alternate Names
TriUbiquitin; Tri-Ubiquitin; Ub3

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