ARA54 Antibody

Novus Biologicals | Catalog # NBP2-15438

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (95%), Mouse (92%), Rat (92%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ARA54 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARA54. The exact sequence is proprietary.

Localization

Cytoplasm; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARA54 Antibody

Western Blot: ARA54 Antibody [NBP2-15438]

Western Blot: ARA54 Antibody [NBP2-15438]

Western Blot: ARA54 Antibody [NBP2-15438] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE; antibody diluted at 1:1000.
ARA54 Antibody

Immunocytochemistry/ Immunofluorescence: ARA54 Antibody [NBP2-15438] -

RNF14 antibody [N1C1] detects RNF14 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RNF14 protein stained by RNF14 antibody [N1C1] (NBP2-15438) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for ARA54 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARA54

The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]

Long Name

Androgen Receptor Associated Protein 54

Alternate Names

HFB30, RNF14, TRIAD2

Gene Symbol

RNF14

UniProt

Q9UBS8

Additional ARA54 Products

Product Documents for ARA54 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ARA54 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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