ARC/NOL3 Antibody

Novus Biologicals | Catalog # NBP2-19561

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ARC Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARC/NOL3. The exact sequence is proprietary.

Localization

Isoform 1: Nucleus; nucleolus; Isoform 2: Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARC/NOL3 Antibody

Immunocytochemistry/ Immunofluorescence: ARC/NOL3 Antibody [NBP2-19561]

Immunocytochemistry/ Immunofluorescence: ARC/NOL3 Antibody [NBP2-19561]

Immunocytochemistry/Immunofluorescence: ARC/NOL3 Antibody [NBP2-19561] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NOL3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561]

Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561]

Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] - Mouse brain. NOL3 stained by NOL3 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561]

Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561]

Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] - Hela xenograft, using NOL3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561]

Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561]

Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] - NOL3 antibody detects NOL3 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NOL3 stained by NOL3 antibody diluted at 1:500.
Immunohistochemistry-Frozen: ARC/NOL3 Antibody [NBP2-19561]

Immunohistochemistry-Frozen: ARC/NOL3 Antibody [NBP2-19561]

Immunohistochemistry-Frozen: ARC/NOL3 Antibody [NBP2-19561] - NOL3 antibody detects NOL3 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum. Green: NOL3 stained by NOL3 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
ARC/NOL3 Antibody

Western Blot: ARC/NOL3 Antibody [NBP2-19561] -

Western Blot: ARC/NOL3 Antibody [NBP2-19561] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ARC/NOL3 antibody (NBP2-19561) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ARC/NOL3 Antibody

Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] -

Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] - ARC/NOL3 antibody detects ARC/NOL3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ARC/NOL3 stained by ARC/NOL3 antibody (NBP2-19561) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ARC/NOL3 Antibody

Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] -

Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] - ARC/NOL3 antibody detects ARC/NOL3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ARC/NOL3 stained by ARC/NOL3 antibody (NBP2-19561) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for ARC/NOL3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARC

This gene encodes an anti-apoptotic protein that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Apoptosis Repressor with Caspase Recruitment Domain

Alternate Names

NOL3

Gene Symbol

NOL3

Additional ARC Products

Product Documents for ARC/NOL3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ARC/NOL3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Cysteine Proteases and Regulators

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail