![Western Blot: ARHGEF16 Antibody [NBP1-06499]](https://resources.rndsystems.com/images/products/ARHGEF16-Antibody-Western-Blot-NBP1-06499-img0003.jpg "Western Blot: ARHGEF16 Antibody [NBP1-06499]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human Rho guanine exchange factor (GEF) 16 using the numbering given in entry NP_055263.2 (GeneID 27237).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ARHGEF16 Antibody
Western Blot: ARHGEF16 Antibody [NBP1-06499]
Western Blot: ARHGEF16 Antibody [NBP1-06499] - Whole cell lysate from HeLa and 293T cells.![Immunohistochemistry-Paraffin: ARHGEF16 Antibody [NBP1-06499]](https://resources.rndsystems.com/images/products/ARHGEF16-Antibody-Immunohistochemistry-Paraffin-NBP1-06499-img0004.jpg "Immunohistochemistry-Paraffin: ARHGEF16 Antibody [NBP1-06499]")
Immunohistochemistry-Paraffin: ARHGEF16 Antibody [NBP1-06499]
Immunohistochemistry-Paraffin: ARHGEF16 Antibody [NBP1-06499] - Human ovarian carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).![Immunohistochemistry-Paraffin: ARHGEF16 Antibody [NBP1-06499]](https://resources.rndsystems.com/images/products/ARHGEF16-Antibody-Immunohistochemistry-Paraffin-NBP1-06499-img0001.jpg "Immunohistochemistry-Paraffin: ARHGEF16 Antibody [NBP1-06499]")
Immunohistochemistry-Paraffin: ARHGEF16 Antibody [NBP1-06499]
Immunohistochemistry-Paraffin: ARHGEF16 Antibody [NBP1-06499] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ARHGEF16 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit![Immunohistochemistry-Paraffin: ARHGEF16 Antibody [NBP1-06499]](https://resources.rndsystems.com/images/products/ARHGEF16-Antibody-Immunohistochemistry-Paraffin-NBP1-06499-img0002.jpg "Immunohistochemistry-Paraffin: ARHGEF16 Antibody [NBP1-06499]")
Immunohistochemistry-Paraffin: ARHGEF16 Antibody [NBP1-06499]
Immunohistochemistry-Paraffin: ARHGEF16 Antibody [NBP1-06499] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ARHGEF16 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.Applications for ARHGEF16 Antibody
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
2-5 mcg/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope exposure is recommended. Epitope exposure with citrate buffer or tris-EDTA pH9.0 buffer will enhance staining. Likely to work with frozen sections
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ARHGEF16
Alternate Names
EPHEXIN4, Ephexin-4, GEF16, NBRRho guanine exchange factor (GEF) 16, Rho guanine nucleotide exchange factor (GEF) 16, rho guanine nucleotide exchange factor 16
Entrez Gene IDs
27237 (Human)
Gene Symbol
ARHGEF16
UniProt
Additional ARHGEF16 Products
Product Documents for ARHGEF16 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ARHGEF16 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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