ARHGEF6 Antibody (2E5) - Azide and BSA Free

Novus Biologicals | Catalog # H00009459-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E5

Format

Azide and BSA Free
View all ARHGEF6 Primary Antibodies »

Product Specifications

Immunogen

ARHGEF6 (AAH39856.1, 61 a.a. ~ 175 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QTEADCINNINDFLKGCATLQVEIFDPDDLYSGVNFSKVLSTLLAVNKATEDQLSERPCGRSSSLSAANTSQTNPQGAVSSTVSGLQRQSKTVEMTENGSHQLIVKARFNFKQTN

Specificity

ARHGEF6 - Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (2E5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ARHGEF6 Antibody (2E5) - Azide and BSA Free

Western Blot: ARHGEF6 Antibody (2E5) [H00009459-M02]

Western Blot: ARHGEF6 Antibody (2E5) [H00009459-M02]

Western Blot: ARHGEF6 Antibody (2E5) [H00009459-M02] - Analysis of ARHGEF6 expression in transfected 293T cell line by ARHGEF6 monoclonal antibody (M02), clone 2E5.Lane 1: ARHGEF6 transfected lysate (Predicted MW: 87.5 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Assay: ARHGEF6 Antibody (2E5) [H00009459-M02]

Proximity Ligation Assay: ARHGEF6 Antibody (2E5) [H00009459-M02]

Proximity Ligation Assay: ARHGEF6 Antibody (2E5) [H00009459-M02] - Analysis of protein-protein interactions between CDC42 and ARHGEF6. HeLa cells were stained with anti-CDC42 rabbit purified polyclonal 1:1200 and anti-ARHGEF6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunoprecipitation: ARHGEF6 Antibody (2E5) [H00009459-M02]

Immunoprecipitation: ARHGEF6 Antibody (2E5) [H00009459-M02]

Immunoprecipitation: ARHGEF6 Antibody (2E5) [H00009459-M02] - Analysis of ARHGEF6 transfected lysate using anti-ARHGEF6 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ARHGEF6 monoclonal antibody.
Sandwich ELISA: ARHGEF6 Antibody (2E5) [H00009459-M02] - Detection limit for recombinant GST tagged ARHGEF6 is approximately 0.03ng/ml as a capture antibody.

Applications for ARHGEF6 Antibody (2E5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARHGEF6

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific mental retardation. [provided by RefSeq]

Alternate Names

alphaPIX, Alpha-Pix, COOL-2, COOL2alpha, KIAA0006Cool2, mental retardation, X-linked 46, MRX46, PAK-interacting exchange factor alpha, PIXA, Rac/Cdc42 guanine exchange factor (GEF) 6, Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6, Rac/Cdc42 guanine nucleotide exchange factor 6, rho guanine nucleotide exchange factor 6

Entrez Gene IDs

9459 (Human)

Gene Symbol

ARHGEF6

UniProt

AAH39856 (Human)

Additional ARHGEF6 Products

Product Documents for ARHGEF6 Antibody (2E5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ARHGEF6 Antibody (2E5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ARHGEF6 Antibody (2E5) - Azide and BSA Free

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Protocols

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FAQs

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