ARID5A Antibody (1683) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43676

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1683

Format

Azide and BSA Free
View all ARID5A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARID5A. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Bovine (88%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARID5A Antibody (1683) - Azide and BSA Free

Immunohistochemistry-Paraffin: ARID5A Antibody (1683) [NBP2-43676]

Immunohistochemistry-Paraffin: ARID5A Antibody (1683) [NBP2-43676]

Immunohistochemistry-Paraffin: ARID5A Antibody (1683) [NBP2-43676] - Mouse urinary bladder. ARID5A stained by ARID5A antibody [1683] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: ARID5A Antibody (1683) [NBP2-43676]

Western Blot: ARID5A Antibody (1683) [NBP2-43676]

Western Blot: ARID5A Antibody (1683) [NBP2-43676] - Analysis of Non-transfected (-) and ARID5A -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody diluted by 1:10000.
Immunohistochemistry-Frozen: ARID5A Antibody (1683) [NBP2-43676]

Immunohistochemistry-Frozen: ARID5A Antibody (1683) [NBP2-43676]

Immunohistochemistry-Frozen: ARID5A Antibody (1683) [NBP2-43676] - detects ARID5A protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse mouse cerebellum. Green: ARID5A stained by ARID5A antibody (1683) diluted at 1:250. Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: ARID5A Antibody (1683) [NBP2-43676]

Immunohistochemistry-Paraffin: ARID5A Antibody (1683) [NBP2-43676]

Immunohistochemistry-Paraffin: ARID5A Antibody (1683) [NBP2-43676] - Mouse brain. ARID5A stained by ARID5A antibody [1683] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
ARID5A Antibody (1683) [NBP2-43676] - ARID5A Antibody (1683) detects ARID5A protein by flow cytometry analysis.Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: ARID5A Antibody (1683) dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
ARID5A Antibody (1683) - Azide and BSA Free

Western Blot: ARID5A Antibody (1683) [NBP2-43676] -

THP-1 whole cell and nuclear extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody [GT1683] (NBP2-43676) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for ARID5A Antibody (1683) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARID5A

Members of the ARID protein family, including ARID5A, have diverse functions but all appear to play important roles indevelopment, tissue-specific gene expression, and regulation of cell growth (Patsialou et al., 2005 (PubMed15640446)).(supplied by OMIM)

Alternate Names

ARID domain-containing protein 5A, AT rich interactive domain 5A (MRF1-like), AT-rich interactive domain-containing protein 5A, RFVG5814

Gene Symbol

ARID5A

Additional ARID5A Products

Product Documents for ARID5A Antibody (1683) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ARID5A Antibody (1683) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ARID5A Antibody (1683) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review ARID5A Antibody (1683) - Azide and BSA Free and earn rewards!

Have you used ARID5A Antibody (1683) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies