ASAH1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15479

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ASAH1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ASAH1. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (100%), Bovine (87%), Rat (84%).

Localization

Lysosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ASAH1 Antibody - BSA Free

Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP2-15479]

Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP2-15479]

Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP2-15479] - Immunohistochemical analysis of paraffin-embedded Adenocarcinoma, using antibody at 1:500 dilution.
ASAH1 Antibody

Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP2-15479] -

Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP2-15479] - ASAH1 antibody detects ASAH1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ASAH1 stained by ASAH1 antibody (NBP2-15479) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ASAH1 Antibody

Western Blot: ASAH1 Antibody [NBP2-15479] -

Western Blot: ASAH1 Antibody [NBP2-15479] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ASAH1 antibody (NBP2-15479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for ASAH1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ASAH1

This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Acid ceramidase

Alternate Names

AC, ACDase, Acid CDase, ASAH, EC 3.5.1.-, EC 3.5.1.23, HSD-33, HSD33

Gene Symbol

ASAH1

UniProt

Q13510 (Human)

Additional ASAH1 Products

Product Documents for ASAH1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ASAH1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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