ASPM Antibody

Novus Biologicals | Catalog # NB100-2278

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Porcine, Bovine, Ovine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ASPM Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 3425 and the C-terminus (residue 3477) of human ASP (Abnormal Spindle)-like, Microcephaly Associated using the numbering given in entry NP_060606.2 (GeneID 259266).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22350525).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASPM Antibody

Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278]

Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278]

Immunocytochemistry/Immunofluorescence: ASPM Antibody [NB100-2278] - Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-ASPM used at a dilution of 1:250. Detection: Goat anti-rabbit IgG (h&l) FITC used at a dilution of 1:100.
Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278]

Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278]

Immunocytochemistry/Immunofluorescence: ASPM Antibody [NB100-2278] - Detection of Human ASPM in NBF-fixed asynchronous HeLa cells with anti-ASPM used at a dilution of 1:250. Detection: Goat anti-rabbit IgG (h&l) FITC used at a dilution of 1:100.

Applications for ASPM Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500
Application Notes
For ICC/IF: Permeabilization with Triton-X 100 is recommended. Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.Likely to work with frozen sections. IHC was reported in scientific literature (PMID: 22350525). Use in IHC-P reported in scientific literature (PMID: 30092011).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.25 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ASPM

The ASPM gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts (Bond et al., 2002). The mouse gene Aspm is expressed specifically in the primary sites of prenatal cerebral cortical neurogenesis.[supplied by OMIM]

Alternate Names

ASP, asp (abnormal spindle) homolog, microcephaly associated (Drosophila), Asp homolog, Calmbp1, FLJ43117, MCPH5DKFZp686N06184, microcephaly associated (Drosophila), microcephaly, primary autosomal recessive 5

Entrez Gene IDs

259266 (Human)

Gene Symbol

ASPM

UniProt

Q8IZT6 (Human)

Additional ASPM Products

Product Documents for ASPM Antibody

Certificate of Analysis

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Product Specific Notices for ASPM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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