Asporin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15492

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Porcine (92%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Asporin. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Asporin Antibody - BSA Free

Western Blot: Asporin Antibody [NBP2-15492]

Western Blot: Asporin Antibody [NBP2-15492]

Asporin-Antibody-Western-Blot-NBP2-15492-img0008.jpg
Immunocytochemistry/ Immunofluorescence: Asporin Antibody [NBP2-15492]

Immunocytochemistry/ Immunofluorescence: Asporin Antibody [NBP2-15492]

Immunocytochemistry/Immunofluorescence: Asporin Antibody [NBP2-15492] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: Asporin Antibody [NBP2-15492]

Western Blot: Asporin Antibody [NBP2-15492]

Western Blot: Asporin Antibody [NBP2-15492] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal diluted at 1:500.
Western Blot: Asporin Antibody [NBP2-15492]

Western Blot: Asporin Antibody [NBP2-15492]

Western Blot: Asporin Antibody [NBP2-15492] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal diluted at 1:500.
Asporin Antibody

Western Blot: Asporin Antibody [NBP2-15492] -

Western Blot: Asporin Antibody [NBP2-15492] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Asporin Antibody

Western Blot: Asporin Antibody [NBP2-15492] -

Comparative immunoblot analysis of normal wild type versus mdx-4cv liver extracts. Shown is a representative silver-stained SDS-PAGE gel & corresponding immunoblots with expanded views of labelling with antibodies to the fatty acid binding protein isoforms FABP1 & FABP5, as well as ferritin light chain, carbonic anhydrase isoform CA3, the voltage-dependent anion channel VDAC-1, the mitochondrial outer membrane protein translocation pore subunit TOM22, fibronectin & asporin. Lanes 1 & 2 represent total extracts from control wild type (wt) liver & mdx-4cv liver, respectively. Molecular mass standards (in kDa) are indicated at the left of the gel image. Graphical representations of the immuno-decoration levels of FABP1 & FABP5 are shown (Y-axis: % control): Student’s t test, unpaired; n = 4; *p < 0.05 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30386187), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Asporin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Asporin

ASPN belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin (MIM 125255) (Lorenzo et al., 2001 [PubMed 11152692]).[supplied by OMIM]

Alternate Names

asporin, asporin (LRR class 1), FLJ20129, OS3, periodontal ligament associated protein 1, Periodontal ligament-associated protein 1, PLAP-1, PLAP1SLRR1Casporin proteoglycan, small leucine-rich protein 1C

Gene Symbol

ASPN

UniProt

Additional Asporin Products

Product Documents for Asporin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Asporin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Asporin Antibody - BSA Free

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Protocols

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FAQs

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