ATAD3A Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00055210-B01P

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Format

Azide and BSA Free
View all ATAD3A Primary Antibodies »

Product Specifications

Immunogen

ATAD3A (AAH33109.1, 1 a.a. - 586 a.a.) full-length human protein. MSWLFGINKGPKGEGAGPPPPLPPAQPGAEGGGDRGLGDRPAPKDKWSNFDPTGLERAAKAARELEHSRYAKDALNLAQMQEQTLQLEQQSKLKEYEAAVEQLKSEQIRAQAEERRKTLSEETRQHQARAQYQDKLARQRYEDQLKQQQLLNEENLRKQEESVQKQEAMRRATVEREMELRHKNEMLRVEAEARARAKAERENADIIREQIRLKAAEHRQTVLESIRTAGTLFGEGFRAFVTDWDKVTATVAGLTLLAVGVYSAKNATLVAGRFIEARLGKPSLVRETSRITVLEALRHPIQVSRRLLSRPQDALEGVVLSPSLEARVRDIAIATRNTKKNRSLYRNILMYGPPGTGKTLFAKKLALHSGMDYAIMTGGDVAPMGREGVTAMHKLFDWANTSRRGLLLFVDEADAFLRKRATEKISEDLRATLNAFLYRTGQHSNKFMLVLASNQPEQFDWAINDRINEMVHFDLPGQEERERLVRMYFDKYVLKPATEGKQRLKLAQFDYGRKCSEVARLTEGMSGREIAQLAVSWQATAYASEDGVLTEAMMDTRVQDAVQQHQQKMCWLKAEGPGRGDEPSPS

Specificity

ATAD3A - ATPase family, AAA domain containing 3A,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for ATAD3A Antibody - Azide and BSA Free

Western Blot: ATAD3A Antibody [H00055210-B01P]

Western Blot: ATAD3A Antibody [H00055210-B01P]

Western Blot: ATAD3A Antibody [H00055210-B01P] - Analysis of ATAD3A expression in human spleen.
Immunocytochemistry/ Immunofluorescence: ATAD3A Antibody [H00055210-B01P]

Immunocytochemistry/ Immunofluorescence: ATAD3A Antibody [H00055210-B01P]

Immunocytochemistry/Immunofluorescence: ATAD3A Antibody [H00055210-B01P] - ATAD3A in human primary fibroblasts. Mitochondrial localization. PFA 4% fixed. Primary incubation 10 ug/mL O/N 4C. Secondary: Donkey anti-mouse Alexafluor 555 1:500 1h RT.
Western Blot: ATAD3A Antibody [H00055210-B01P]

Western Blot: ATAD3A Antibody [H00055210-B01P]

Western Blot: ATAD3A Antibody [H00055210-B01P] - Analysis of ATAD3A expression in HeLa.
Western Blot: ATAD3A Antibody [H00055210-B01P]

Western Blot: ATAD3A Antibody [H00055210-B01P]

Western Blot: ATAD3A Antibody [H00055210-B01P] - Analysis of ATAD3A expression in transfected 293T cell line by ATAD3A polyclonal antibody. Lane 1: ATAD3A transfected lysate(64.46 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: ATAD3A Antibody [H00055210-B01P]

Immunocytochemistry/ Immunofluorescence: ATAD3A Antibody [H00055210-B01P]

Immunocytochemistry/Immunofluorescence: ATAD3A Antibody [H00055210-B01P] - Analysis of purified antibody to ATAD3A on HeLa cell. (antibody concentration 10 ug/ml)

Applications for ATAD3A Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in Western blot. GST tag alone is used as a negative control.

Reviewed Applications

Read 2 reviews rated 5 using H00055210-B01P in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATAD3A

ATAD3A and ATAD3B (MIM 612317) are mitochondrial membrane proteins that contribute to the stabilization of large mitochondrial DNA (mtDNA)-protein complexes called nucleoids (He et al., 2007 [PubMed 17210950]).[supplied by OMIM]

Alternate Names

ATPase family AAA domain-containing protein 3A, ATPase family, AAA domain containing 3A, FLJ10709, FLJ35514

Entrez Gene IDs

55210 (Human)

Gene Symbol

ATAD3A

UniProt

AAH33109.1 (Human)

Additional ATAD3A Products

Product Documents for ATAD3A Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ATAD3A Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ATAD3A Antibody - Azide and BSA Free (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • ATAD3A Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human primary fibroblast
    Species: Human
    Verified Customer | Posted 11/08/2020
    Abnova ab ATAD3A. TGX 4-15% gel. Denaturalized and reductive conditions. Blocking Milk 5% in TBST (0.1% Tween). Primary ab incubation 1:1000 in Milk 5% in TBST (0.1% Tweeen) ON 4ºC. Secondary 1:5000 Goat anti-mouse alexa fluor 647.
    Abnova ab ATAD3A. TGX 4-15% gel. Denaturalized and reductive conditions. Blocking Milk 5% in TBST (0.1% Tween). Primary ab incubation 1:1000 in Milk 5% in TBST (0.1% Tweeen) ON 4ºC. Secondary 1:5000 Goat anti-mouse alexa fluor 647 1h RT.
    ATAD3A Antibody - Azide and BSA Free H00055210-B01P
  • ATAD3A Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human primary fibroblast
    Species: Human
    Verified Customer | Posted 11/08/2020
    ATAD3A in human primary fibroblasts. Mitochondrial localization. PFA 4% fixed. Primary incubation 10 ug/ml O/N 4ºC. Secondary: Donkey anti-mouse Alexafluor 555 1:500 1h RT.
    Not checked if it detects only ATAD3A. Human primary fibroblasts PFA 4% fixed. Blocking 1h DG 10% in PBST (0.1% Triton). Primary incubation 10 ug/ml O/N 4ºC. Secondary: Donkey anti-mouse Alexafluor 555 1:500 1h RT.
    ATAD3A Antibody - Azide and BSA Free H00055210-B01P

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Protocols

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