ATF4 Antibody (2B3) - Azide and BSA Free

Novus Biologicals | Catalog # H00000468-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2B3

Format

Azide and BSA Free
View all ATF4 Primary Antibodies »

Product Specifications

Immunogen

ATF4 (NP_001666.2, 171 a.a. ~ 270 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SSTPDHSFSLELGSEVDITEGDRKPDYTAYVAMIPQCIKEEDTPSDNDSGICMSPESYLGSPQHSPSTRGSPNRSLPSPGVLCGSARPKPYDPPGEKMVA

Reactivity Notes

Mouse reported in scientific literature (PMID: 23223004).

Specificity

ATF4 - activating transcription factor 4 (tax-responsive enhancer element B67)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ATF4 Antibody (2B3) - Azide and BSA Free

Western Blot: ATF4 Antibody (2B3) [H00000468-M01]

Western Blot: ATF4 Antibody (2B3) [H00000468-M01]

Western Blot: ATF4 Antibody (2B3) [H00000468-M01] - Analysis of ATF4 expression in transfected 293T cell line by ATF4 monoclonal antibody (M01), clone 2B3.Lane 1: ATF4 transfected lysate(38.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (2B3) [H00000468-M01]

Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (2B3) [H00000468-M01]

Immunocytochemistry/Immunofluorescence: ATF4 Antibody (2B3) [H00000468-M01] - Analysis of monoclonal antibody to ATF4 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: ATF4 Antibody (2B3) [H00000468-M01]

Western Blot: ATF4 Antibody (2B3) [H00000468-M01]

Western Blot: ATF4 Antibody (2B3) [H00000468-M01] - Western Blot analysis of ATF4 expression in NIH/3T3 ( Cat # L018V1 ).
ELISA: ATF4 Antibody (2B3) [H00000468-M01]

ELISA: ATF4 Antibody (2B3) [H00000468-M01]

ELISA: ATF4 Antibody (2B3) [H00000468-M01] - Detection limit for recombinant GST tagged ATF4 is approximately 0.03ng/ml as a capture antibody.

Applications for ATF4 Antibody (2B3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATF4

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

Long Name

Activating Transcription Factor 4

Alternate Names

TAXREB67, TXREB

Entrez Gene IDs

468 (Human); 11911 (Mouse)

Gene Symbol

ATF4

OMIM

604064 (Human)

UniProt

P18848 (Human)

Additional ATF4 Products

Product Documents for ATF4 Antibody (2B3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ATF4 Antibody (2B3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ATF4 Antibody (2B3) - Azide and BSA Free

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Protocols

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail