ATF4 Antibody (S360A-24) - BSA Free

Novus Biologicals | Catalog # NBP2-42176

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # S360A-24

Format

BSA Free
View all ATF4 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 25-327 of human ATF4. 86% identical to rat, and 85% identical to mouse. 50% identity with ATF5.

Localization

Cytoplasm, Cell Membrane, Nucleus

Specificity

Less then 50% identity with ATF5.Detects approx 60kDa. Does not cross-react with ATF5.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for ATF4 Antibody (S360A-24) - BSA Free

Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176]

Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176]

Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176] - Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4protein using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).
Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176]

Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176]

Immunocytochemistry/Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) ATF4Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176]

Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176]

Immunocytochemistry/Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Cell Membrane. Magnification: 60X.
ATF4 Antibody (S360A-24) - BSA Free

Western Blot: ATF4 Antibody (S360A-24) - BSA Free [NBP2-42176] -

FABP4 inhibitor BMS309403 attenuates ER stress in cardiomyocytes exposed to hypoxia. (A, B) H9c2 cardiomyocytes were cultured under normoxia or hypoxia condition for 24 h in the presence or absence of 50 uM BMS309403. The protein expressions of GPR78, CHOP and caspase‐12 (A), and p‐PERK, PERK, p‐eIF2 alpha, eIF2 and ATF4 (B) were determined by Western blot analysis. beta ‐actin is the internal control. The quantification analysis of protein expression is shown in the right. Student's t test (n = 3). **, P < 0.01 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32896039), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ATF4 Antibody (S360A-24) - BSA Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
1 ug/ml of ATF4 Antibody was sufficient for detection of ATF4 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATF4

ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

Long Name

Activating Transcription Factor 4

Alternate Names

TAXREB67, TXREB

Gene Symbol

ATF4

UniProt

NP_001666.2 (Human), P18848 (Human)

Additional ATF4 Products

Product Documents for ATF4 Antibody (S360A-24) - BSA Free

Certificate of Analysis

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Product Specific Notices for ATF4 Antibody (S360A-24) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ATF4 Antibody (S360A-24) - BSA Free

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Protocols

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail