ATF4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15499

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (90%), Chimpanzee (99%), Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATF4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATF4. The exact sequence is proprietary.

Reactivity Notes

Pig (89%), Sheep (86%), Bovine (86%).

Localization

Cytoplasm; Cell membrane; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit ATF4 Antibody - BSA Free (NBP2-15499) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-ATF4 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ATF4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ATF4 Antibody [NBP2-15499]

Immunocytochemistry/ Immunofluorescence: ATF4 Antibody [NBP2-15499]

Immunocytochemistry/Immunofluorescence: ATF4 Antibody [NBP2-15499] - HeLa cells mock (left) and treated with 2 ug/ml Tunicamycin for 8hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATF4 protein stained by ATF4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
ATF4 Antibody - BSA Free

Western Blot: ATF4 Antibody [NBP2-15499] -

Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF4 antibody (NBP2-15499) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for ATF4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATF4

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication. [provided by RefSeq]

Long Name

Activating Transcription Factor 4

Alternate Names

TAXREB67, TXREB

Gene Symbol

ATF4

UniProt

P18848 (Human)

Additional ATF4 Products

Product Documents for ATF4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ATF4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ATF4 Antibody - BSA Free

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Protocols

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail