ATG13 Antibody (3E4C8) - BSA Free

Novus Biologicals | Catalog # NBP2-52419

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3E4C8

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human ATG13 (AA: 339-550) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATG13 Antibody (3E4C8) - BSA Free

Western Blot: ATG13 Antibody (3E4C8)BSA Free [NBP2-52419]

Western Blot: ATG13 Antibody (3E4C8)BSA Free [NBP2-52419]

Western Blot: ATG13 Antibody (3E4C8) [NBP2-52419] - Analysis using ATG13 mAb against HEK293 (1) and ATG13 (AA: 339-550)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: ATG13 Antibody (3E4C8) - BSA Free [NBP2-52419]

Immunocytochemistry/ Immunofluorescence: ATG13 Antibody (3E4C8) - BSA Free [NBP2-52419]

Immunocytochemistry/Immunofluorescence: ATG13 Antibody (3E4C8) [NBP2-52419] - Immunofluorescence analysis of Hela cells using ATG13 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Flow Cytometry: ATG13 Antibody (3E4C8) - BSA Free [NBP2-52419]

Flow Cytometry: ATG13 Antibody (3E4C8) - BSA Free [NBP2-52419]

Flow Cytometry: ATG13 Antibody (3E4C8) [NBP2-52419] - Analysis of Hela cells using ATG13 mouse mAb (green) and negative control (red).
Western Blot: ATG13 Antibody (3E4C8)BSA Free [NBP2-52419]

Western Blot: ATG13 Antibody (3E4C8)BSA Free [NBP2-52419]

Western Blot: ATG13 Antibody (3E4C8) [NBP2-52419] - Western blot analysis using ATG13 mAb against human ATG13 (AA: 339-550) recombinant protein. (Expected MW is 48.9 kDa)
ELISA: ATG13 Antibody (3E4C8) - BSA Free [NBP2-52419]

ELISA: ATG13 Antibody (3E4C8) - BSA Free [NBP2-52419]

ELISA: ATG13 Antibody (3E4C8) [NBP2-52419] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Applications for ATG13 Antibody (3E4C8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG13

Alternate Names

autophagy related 13, autophagy-related protein 13, KIAA0652

Gene Symbol

ATG13

Additional ATG13 Products

Product Documents for ATG13 Antibody (3E4C8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ATG13 Antibody (3E4C8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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