ATG13 Antibody (6B2E12) - BSA Free
Novus Biologicals | Catalog # NBP2-52418
![Western Blot: ATG13 Antibody (6B2E12)BSA Free [NBP2-52418]](https://resources.rndsystems.com/images/products/ATG13-Antibody-6B2E12-Western-Blot-NBP2-52418-img0005.jpg "Western Blot: ATG13 Antibody (6B2E12)BSA Free [NBP2-52418]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 6B2E12
Format
BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of human ATG13 (AA: 339-550) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
56.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ATG13 Antibody (6B2E12) - BSA Free
Western Blot: ATG13 Antibody (6B2E12)BSA Free [NBP2-52418]
Western Blot: ATG13 Antibody (6B2E12) [NBP2-52418] - Analysis using ATG13 mAb against (1) HEK293 and (2) ATG13 (AA: 339-550)-hIgGFc transfected HEK293 cell lysate.![Immunohistochemistry-Paraffin: ATG13 Antibody (6B2E12) - BSA Free [NBP2-52418]](https://resources.rndsystems.com/images/products/ATG13-Antibody-6B2E12-Immunohistochemistry-Paraffin-NBP2-52418-img0003.jpg "Immunohistochemistry-Paraffin: ATG13 Antibody (6B2E12) - BSA Free [NBP2-52418]")
Immunohistochemistry-Paraffin: ATG13 Antibody (6B2E12) - BSA Free [NBP2-52418]
Immunohistochemistry-Paraffin: ATG13 Antibody (6B2E12) [NBP2-52418] - Analysis of paraffin-embedded striated muscle tissues using ATG13 mouse mAb with DAB staining.![Western Blot: ATG13 Antibody (6B2E12)BSA Free [NBP2-52418]](https://resources.rndsystems.com/images/products/ATG13-Antibody-6B2E12-Western-Blot-NBP2-52418-img0002.jpg "Western Blot: ATG13 Antibody (6B2E12)BSA Free [NBP2-52418]")
Western Blot: ATG13 Antibody (6B2E12)BSA Free [NBP2-52418]
Western Blot: ATG13 Antibody (6B2E12) [NBP2-52418] - Analysis using ATG13 mAb against human ATG13 (AA: 339-550) recombinant protein. (Expected MW is 48.9 kDa)![Immunohistochemistry: ATG13 Antibody (6B2E12) - BSA Free [NBP2-52418]](https://resources.rndsystems.com/images/products/ATG13-Antibody-6B2E12-Immunohistochemistry-NBP2-52418-img0001.jpg "Immunohistochemistry: ATG13 Antibody (6B2E12) - BSA Free [NBP2-52418]")
Immunohistochemistry: ATG13 Antibody (6B2E12) - BSA Free [NBP2-52418]
Immunohistochemistry: ATG13 Antibody (6B2E12) [NBP2-52418] - Immunohistochemical analysis of paraffin-embedded cardiac muscle tissues using ATG13 mouse mAb with DAB staining.![ELISA: ATG13 Antibody (6B2E12) - BSA Free [NBP2-52418]](https://resources.rndsystems.com/images/products/ATG13-Antibody-6B2E12-ELISA-NBP2-52418-img0004.jpg "ELISA: ATG13 Antibody (6B2E12) - BSA Free [NBP2-52418]")
ELISA: ATG13 Antibody (6B2E12) - BSA Free [NBP2-52418]
ELISA: ATG13 Antibody (6B2E12) [NBP2-52418] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).Applications for ATG13 Antibody (6B2E12) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Application Notes
This antibody is Cytof ready.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ATG13
Alternate Names
autophagy related 13, autophagy-related protein 13, KIAA0652
Gene Symbol
ATG13
Additional ATG13 Products
Product Documents for ATG13 Antibody (6B2E12) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ATG13 Antibody (6B2E12) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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