ATG4B Antibody (9H6M6)

Novus Biologicals | Catalog # NBP3-16538

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9H6M6 expressed in HEK293
View all ATG4B Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ATG4B (Q9Y4P1). DRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATG4B Antibody (9H6M6)

Western Blot: ATG4B Antibody (9H6M6) [NBP3-16538]

Western Blot: ATG4B Antibody (9H6M6) [NBP3-16538]

Western Blot: ATG4B Antibody (9H6M6) [NBP3-16538] - Western blot analysis of extracts of various cell lines, using ATG4B Rabbit mAb (NBP3-16538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538]

Immunocytochemistry/ Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538]

Immunocytochemistry/Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538] - Immunofluorescence analysis of NIH-3T3 cells using ATG4B Rabbit mAb (NBP3-16538) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ATG4B Antibody (9H6M6)

Immunocytochemistry/ Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538] -

Immunocytochemistry/ Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538] - Confocal imaging of NIH/3T3 cells using ATG4B Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ATG4B Antibody (9H6M6)

Immunocytochemistry/ Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538] -

Immunocytochemistry/ Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538] - Confocal imaging of paraffin-embedded Mouse brain using ATG4B Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). Objective: 40x. DAPI was used for nuclear staining (Blue). Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Applications for ATG4B Antibody (9H6M6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATG4B

FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 5 named isoforms produced by alternative splicing.

Long Name

ATG4 Autophagy Related 4 Homolog B

Alternate Names

Apg4B, AUTL1

Gene Symbol

ATG4B

Additional ATG4B Products

Product Documents for ATG4B Antibody (9H6M6)

Certificate of Analysis

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Product Specific Notices for ATG4B Antibody (9H6M6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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