ATG4D Antibody

Novus Biologicals | Catalog # NBP1-36939

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ATG4D Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CSQLAQPTLRLPRT corresponding to internal region (near C-Terminus) according to NP_116274.3.

Epitope

internal region (near C terminus)

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Dog, Pig

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ATG4D Antibody

ATG4D Antibody

Immunocytochemistry/Immunofluorescence: Goat Polyclonal ATG4D Antibody [NBP1-36939] -

Immunocytochemistry/Immunofluorescence: Goat Polyclonal ATG4D Antibody [NBP1-36939] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-36939]

Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-36939]

Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-36939] - Staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ATG4D Antibody

Flow Cytometry: Goat Polyclonal ATG4D Antibody [NBP1-36939] -

Flow Cytometry: Goat Polyclonal ATG4D Antibody [NBP1-36939] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for ATG4D Antibody

Application
Recommended Usage

ELISA

Detection limit 1:32000

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml
Application Notes
Western blot: Preliminary experiments gave an approx. 26kDa band in Human Skeletal Muscle lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 52.9kDa according to NP_116274.3. The 26kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Pancreas

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATG4D

ATG4D is a gene that codes for a protein expressed in the skeletal muscle and testis and is 474 amino acids long with a weight of approximately 53 kDa. ATG4D is a cysteine protease required for autophagy. ATG4D has been shown to have interactions with TAF12, CASP3, CASP8, GABARAP, and UBC in pathways such as the regulation of autophagy pathway.

Alternate Names

APG4 autophagy 4 homolog D, APG4 autophagy 4 homolog D (S. cerevisiae), APG4D, APG4-D, ATG4 autophagy related 4 homolog D (S. cerevisiae), AUTL4, AUT-like 4 cysteine endopeptidase, AUT-like 4, cysteine endopeptidase, AUT-like 4, cysteine endopeptidase (S. cerevisiae), autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, cysteine protease ATG4D, cysteine protease involved in autophagy, EC 3.4.22, EC 3.4.22.-

Entrez Gene IDs

84971 (Human); 235040 (Mouse); 686505 (Rat)

Gene Symbol

ATG4D

UniProt

NP_116274.3 (Human)

Additional ATG4D Products

Product Documents for ATG4D Antibody

Certificate of Analysis

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Product Specific Notices for ATG4D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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