ATG9B Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-13108

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATG9B Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ATG9B. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

101 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for ATG9B Antibody - BSA Free

Western Blot: ATG9B Antibody [NBP3-13108]

Western Blot: ATG9B Antibody [NBP3-13108]

Western Blot: ATG9B Antibody [NBP3-13108] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG9B antibody (NBP3-13108) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: ATG9B Antibody [NBP3-13108]

Immunocytochemistry/ Immunofluorescence: ATG9B Antibody [NBP3-13108]

Immunocytochemistry/Immunofluorescence: ATG9B Antibody [NBP3-13108] - ATG9B antibody detects ATG9B protein at cytoplasm by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 500 uM Chloroquine for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG9B protein stained by ATG9B antibody (NBP3-13108) diluted at 1:500. Blue: Hoechst 33342 staining.

Applications for ATG9B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG9B

FUNCTION: May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. SUBUNIT: Interacts with GABRG2, TUBA1, ULK1 and NSF. Interacts with beta-tubulin and GPHN. SUBCELLULAR LOCATION: Intracytoplasmic membrane. Cytoskeleton. Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules. SIMILARITY: Belongs to the MAP1 LC3 family.

Alternate Names

APG9L2APG9-like 2, ATG9 autophagy related 9 homolog B (S. cerevisiae), autophagy-related protein 9B, endothelial nitric oxide synthase antisense, nitric oxide synthase 3 antisense, Nitric oxide synthase 3-overlapping antisense gene protein, NOS3ASautophagy 9-like 2 protein, Protein sONE, SONEFLJ14885

Gene Symbol

ATG9B

Additional ATG9B Products

Product Documents for ATG9B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ATG9B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for ATG9B Antibody - BSA Free

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  • Q: How much is the molecular weight of ATG9B protein?

    A: The predicted molecular weight of ATG9B protein is 101kDa

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