ATP Citrate Lyase Antibody (8A0V0)

Novus Biologicals | Catalog # NBP3-16261

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8A0V0 expressed in HEK293
View all ATP Citrate Lyase Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1000-1101 of human ATP Citrate Lyase (P53396). ATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRNCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

121 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATP Citrate Lyase Antibody (8A0V0)

Western Blot: ATP Citrate Lyase Antibody (8A0V0) [NBP3-16261]

Western Blot: ATP Citrate Lyase Antibody (8A0V0) [NBP3-16261]

Western Blot: ATP Citrate Lyase Antibody (8A0V0) [NBP3-16261] - Western blot analysis of extracts of various cell lines, using ATP Citrate Lyase Rabbit mAb (NBP3-16261) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
ATP Citrate Lyase Antibody (8A0V0)

Immunocytochemistry/ Immunofluorescence: ATP Citrate Lyase Antibody (8A0V0) [NBP3-16261] -

Immunocytochemistry/ Immunofluorescence: ATP Citrate Lyase Antibody (8A0V0) [NBP3-16261] - Confocal imaging of U-2 OS cells using ATP Citrate Lyase Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
ATP Citrate Lyase Antibody (8A0V0)

Immunocytochemistry/ Immunofluorescence: ATP Citrate Lyase Antibody (8A0V0) [NBP3-16261] -

Immunocytochemistry/ Immunofluorescence: ATP Citrate Lyase Antibody (8A0V0) [NBP3-16261] - Confocal imaging of NIH/3T3 cells using ATP Citrate Lyase Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
ATP Citrate Lyase Antibody (8A0V0)

Immunoprecipitation: ATP Citrate Lyase Antibody (8A0V0) [NBP3-16261] -

Immunoprecipitation: ATP Citrate Lyase Antibody (8A0V0) [NBP3-16261] - Immunoprecipitation analysis of 300 ug extracts from HepG2 cells using 3 ug ATP Citrate Lyase antibody. Western blot was performed from the immunoprecipitate using ATP Citrate Lyase antibody at a dilution of 1:1000.

Applications for ATP Citrate Lyase Antibody (8A0V0)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells.

Western Blot

1:6000 - 1:20000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATP Citrate Lyase

ATP citrate lyase (ACLY), also known as ATP citrate synthase, is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACLY and insulin to increase phosphorylation of ACLY (2).

Long Name

ATP-citrate synthase

Alternate Names

ACLY, EC 2.3.3.8

Gene Symbol

ACLY

Additional ATP Citrate Lyase Products

Product Documents for ATP Citrate Lyase Antibody (8A0V0)

Certificate of Analysis

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Product Specific Notices for ATP Citrate Lyase Antibody (8A0V0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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