ATP5A Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15512

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (96%), Canine (95%), Chicken (90%), Chimpanzee (100%), Porcine (96%), Rhesus Macaque (98%), Zebrafish (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATP5A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ATP5A. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (89%).

Localization

Mitochondrion inner membrane

Marker

Mitochondria Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATP5A Antibody - BSA Free

Western Blot: ATP5A Antibody [NBP2-15512]

Western Blot: ATP5A Antibody [NBP2-15512]

Western Blot: ATP5A Antibody [NBP2-15512] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: ATP5A Antibody [NBP2-15512]

Western Blot: ATP5A Antibody [NBP2-15512]

Western Blot: ATP5A Antibody [NBP2-15512] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:1000.
Western Blot: ATP5A Antibody [NBP2-15512]

Western Blot: ATP5A Antibody [NBP2-15512]

Western Blot: ATP5A Antibody [NBP2-15512] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody diluted by 1:2000.
Western Blot: ATP5A Antibody [NBP2-15512]

Western Blot: ATP5A Antibody [NBP2-15512]

Western Blot: ATP5A Antibody [NBP2-15512] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: ATP5A Antibody [NBP2-15512]

Western Blot: ATP5A Antibody [NBP2-15512]

Western Blot: ATP5A Antibody [NBP2-15512] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term (diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512]

Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512]

Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Colon carcinoma. ATP5A1 antibody [C2C3], C-term dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512]

Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512]

Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Mouse stomach. ATP5A1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512]

Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512]

Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Mouse lung. ATP5A1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
ATP5A Antibody - BSA Free

Immunohistochemistry-Frozen: ATP5A Antibody [NBP2-15512] -

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term (NBP2-15512) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI.
ATP5A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ATP5A Antibody [NBP2-15512] -

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term (NBP2-15512) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for ATP5A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP2-15512 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATP5A

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the same protein have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq]

Long Name

ATP5A1

Alternate Names

ATP synthase alpha chain, mitochondrial, ATP synthase subunit alpha, mitochondrial, ATP synthase, H+ transporting, mitochondrial F1 co, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform1, cardiac muscle, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform2, non-cardiac muscle-like 2, ATP sythase (F1-ATPase) alpha subunit, ATP5A, ATP5A1, ATP5AL2, ATP5AMOM2, ATPM, ATPMATP synthase subunit alpha, mitochondrial, cardiac muscle, COXPD22, EC 3.6.3, EC 3.6.3.14, epididymis secretory sperm binding protein Li 123m, hATP1, HEL-S-123m, MC5DN4, MC5DN4A, MC5DN4B, mitochondrial ATP synthetase, oligomycin-resistant, MOM2, OMR, ORM, ORMATP synthase alpha chain, mitochondrial

Gene Symbol

ATP5F1A

UniProt

P25705 (Human)

Additional ATP5A Products

Product Documents for ATP5A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ATP5A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ATP5A Antibody - BSA Free

Customer Reviews for ATP5A Antibody - BSA Free (1)

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  • ATP5A Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: glioblastoma cell lysates
    Species: Human
    Verified Customer | Posted 07/15/2023
    used glioma cell line. left--> 1.Ct, 2.drug, 3.Ct, 4. drug (1,2) and (3,4) are different cell line.
    ATP5A Antibody - BSA Free NBP2-15512

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