ATP5J Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15514

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATP5J Primary Antibodies »

Product Specifications

Immunogen

Full length human ATP5J Recombinant protein.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%).

Localization

Mitochondrion; Mitochondrion inner membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATP5J Antibody - BSA Free

Western Blot: ATP5J Antibody [NBP2-15514]

Western Blot: ATP5J Antibody [NBP2-15514]

Western Blot: ATP5J Antibody [NBP2-15514] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa membrane 15% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: ATP5J Antibody [NBP2-15514]

Immunocytochemistry/ Immunofluorescence: ATP5J Antibody [NBP2-15514]

Immunocytochemistry/Immunofluorescence: ATP5J Antibody [NBP2-15514] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: ATP5J Antibody [NBP2-15514]

Immunohistochemistry-Paraffin: ATP5J Antibody [NBP2-15514]

Immunohistochemistry-Paraffin: ATP5J Antibody [NBP2-15514] - Immunohistochemical analysis of paraffin-embedded J5 xenograft, using antibody at 1:500 dilution.
ATP5J Antibody - BSA Free

Western Blot: ATP5J Antibody [NBP2-15514] -

Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody (NBP2-15514) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ATP5J Antibody - BSA Free

Western Blot: ATP5J Antibody [NBP2-15514] -

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody (NBP2-15514) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ATP5J Antibody - BSA Free

Western Blot: ATP5J Antibody [NBP2-15514] -

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody (NBP2-15514) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for ATP5J Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATP5J

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the F0 complex, required for F1 and F0 interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

Alternate Names

ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6, ATP5, ATP5A, ATPase subunit F6, ATPM, coupling factor 6, F6, mitochondrial, mitochondrial ATP synthase, coupling factor 6, mitochondrial ATP synthase, subunit F6, mitochondrial ATPase coupling factor 6, proliferation-inducing protein 36

Gene Symbol

ATP5PF

UniProt

P18859 (Human)

Additional ATP5J Products

Product Documents for ATP5J Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ATP5J Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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