ATP6V0D1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88893

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATP6V0D1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TELSKEDRAKLFPHCGRLYPEGLAQLARADDYEQVKNVADYYPEYKLLFEGAGSNPGDKTLEDRFFEHEVKLNKLAFLNQFHFGVFYAFVKL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATP6V0D1 Antibody - BSA Free

Western Blot: ATP6V0D1 Antibody [NBP1-88893]

Western Blot: ATP6V0D1 Antibody [NBP1-88893]

Western Blot: ATP6V0D1 Antibody [NBP1-88893] - Analysis in human cell line MCF-7.
Immunohistochemistry-Paraffin: ATP6V0D1 Antibody [NBP1-88893]

Immunohistochemistry-Paraffin: ATP6V0D1 Antibody [NBP1-88893]

Immunohistochemistry-Paraffin: ATP6V0D1 Antibody [NBP1-88893] - Staining of human kidney shows distinct positivity in extracellular material.
Western Blot: ATP6V0D1 Antibody [NBP1-88893]

Western Blot: ATP6V0D1 Antibody [NBP1-88893]

Western Blot: ATP6V0D1 Antibody [NBP1-88893] - Analysis in control (vector only transfected HEK293T lysate) and ATP6V0D1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Applications for ATP6V0D1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP6V0D1

ATP6V0D1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously.

Alternate Names

32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0, ATPase, H+ transporting, lysosomal (vacuolar proton pump), member D, ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d isoform 1, ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1, FLJ43534, H(+)-transporting two-sector ATPase, subunit D, p39, Vacuolar proton pump subunit d 1, V-ATPase 40 kDa accessory protein, V-ATPase AC39 subunit, V-ATPase, subunit D, VATX, Vma6, VPATPDV-ATPase subunit d 1, V-type proton ATPase subunit d 1

Gene Symbol

ATP6V0D1

Additional ATP6V0D1 Products

Product Documents for ATP6V0D1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ATP6V0D1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ATP6V0D1 Antibody - BSA Free

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Protocols

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