ATP6V1A Antibody (24GB6940)

Novus Biologicals | Catalog # NBP3-51804

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB6940
View all ATP6V1A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of human ATP6V1A (Uniprot: P38606)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATP6V1A Antibody (24GB6940)

ATP6V1A Antibody (24GB6940)

Immunocytochemistry/ Immunofluorescence: ATP6V1A Antibody (24GB6940)[NBP3-51804] -

Immunocytochemical staining of HepG2 cells with ATP6V1A antibody (1:1,000). Nuclei were stained blue with DAPI; ATP6V1A was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
ATP6V1A Antibody (24GB6940)

Flow Cytometry: ATP6V1A Antibody (24GB6940)[NBP3-51804] -

Flow cytometric analysis of ATP6V1Aexpression in HepG2 cells using ATP6V1A antibody (1:2,000). Green, isotype control; red, ATP6V1A.
ATP6V1A Antibody (24GB6940)

Flow Cytometry: ATP6V1A Antibody (24GB6940)[NBP3-51804] -

Validation of ATP6V1A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with ATP6V1A antibody (1:2,000) and analyzed using BD flow cytometer.

Applications for ATP6V1A Antibody (24GB6940)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATP6V1A

ATP6V1A encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq]

Long Name

V-type proton ATPase catalytic subunit A

Alternate Names

ATP6A1, ATP6V1A1, EC 7.1.2.2, V-ATPase subunit A, VPP2

Gene Symbol

ATP6V1A

Additional ATP6V1A Products

Product Documents for ATP6V1A Antibody (24GB6940)

Certificate of Analysis

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Product Specific Notices for ATP6V1A Antibody (24GB6940)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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