ATP6V1B1 Antibody (OTI1H6)

Novus Biologicals | Catalog # NBP2-00580

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1H6
View all ATP6V1B1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ATP6V1B1 (NP_001683) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATP6V1B1 Antibody (OTI1H6)

Western Blot: ATP6V1B1 Antibody (OTI1H6) [NBP2-00580]

Western Blot: ATP6V1B1 Antibody (OTI1H6) [NBP2-00580]

Western Blot: ATP6V1B1 Antibody (1H6) [NBP2-00580] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1B1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1B1.
Immunocytochemistry/Immunofluorescence: ATP6V1B1 Antibody (1H6) [NBP2-00580] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP6V1B1.
Immunohistochemistry-Paraffin: ATP6V1B1 Antibody (OTI1H6) [NBP2-00580]

Immunohistochemistry-Paraffin: ATP6V1B1 Antibody (OTI1H6) [NBP2-00580]

Immunohistochemistry-Paraffin: ATP6V1B1 Antibody (1H6) [NBP2-00580] - Staining of paraffin-embedded Human Kidney tissue using anti-ATP6V1B1 mouse monoclonal antibody.
Flow Cytometry: ATP6V1B1 Antibody (OTI1H6) [NBP2-00580]

Flow Cytometry: ATP6V1B1 Antibody (OTI1H6) [NBP2-00580]

Flow Cytometry: ATP6V1B1 Antibody (1H6) [NBP2-00580] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATP6V1B1 antibody, and then analyzed by flow cytometry.
Immunohistochemistry-Paraffin: ATP6V1B1 Antibody (OTI1H6) [NBP2-00580]

Immunohistochemistry-Paraffin: ATP6V1B1 Antibody (OTI1H6) [NBP2-00580]

Immunohistochemistry-Paraffin: ATP6V1B1 Antibody (1H6) [NBP2-00580] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATP6V1B1 mouse monoclonal antibody.
Flow Cytometry: ATP6V1B1 Antibody (OTI1H6) [NBP2-00580]

Flow Cytometry: ATP6V1B1 Antibody (OTI1H6) [NBP2-00580]

Flow Cytometry: ATP6V1B1 Antibody (1H6) [NBP2-00580] - Analysis of Hela cells, using anti-ATP6V1B1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: ATP6V1B1 Antibody (OTI1H6) [NBP2-00580]

Flow Cytometry: ATP6V1B1 Antibody (OTI1H6) [NBP2-00580]

Flow Cytometry: ATP6V1B1 Antibody (1H6) [NBP2-00580] - Analysis of Jurkat cells, using anti-ATP6V1B1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for ATP6V1B1 Antibody (OTI1H6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATP6V1B1

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq].

Alternate Names

ATP6B158kD subunit, ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1, Endomembrane proton pump 58 kDa subunit, H+-ATPase beta 1 subunit, vacuolar proton pump 3, Vacuolar proton pump subunit B 1, vacuolar proton pump, subunit 3, VATBMGC32642, V-ATPase B1 subunit, V-ATPase subunit B 1, Vma2, V-type proton ATPase subunit B, kidney isoform

Entrez Gene IDs

525 (Human)

Gene Symbol

ATP6V1B1

Additional ATP6V1B1 Products

Product Documents for ATP6V1B1 Antibody (OTI1H6)

Certificate of Analysis

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Product Specific Notices for ATP6V1B1 Antibody (OTI1H6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ATP6V1B1 Antibody (OTI1H6)

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Protocols

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