Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATPase Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human ATPase fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ATPase Antibody - BSA Free

Immunohistochemistry-Paraffin: ATPase Antibody [NBP2-98188]

Immunohistochemistry-Paraffin: ATPase Antibody [NBP2-98188]

Immunohistochemistry-Paraffin: ATPase Antibody [NBP2-98188] - Immunochemical staining of human ATPase in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: ATPase Antibody [NBP2-98188]

Immunohistochemistry-Paraffin: ATPase Antibody [NBP2-98188]

Immunohistochemistry-Paraffin: ATPase Antibody [NBP2-98188] - Immunochemical staining of human ATPase in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Applications for ATPase Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATPase

CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (By similarity). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the cation transport ATPase (P-type) family. Type V subfamily.

Alternate Names

ATPase, axonemal, dynein, axonemal, heavy chain 8, dynein, axonemal, heavy polypeptide 8, FLJ25850, FLJ36115, FLJ36334, HDHC9

Gene Symbol

DNAH8

Additional ATPase Products

Product Documents for ATPase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ATPase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

ATPases

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ATPase Antibody - BSA Free

Showing  1 - 4 of 4 FAQs Showing All

  • Q: I have phosphate in my enzyme. What can I do?

    A: You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.

  • Q: I have 5% DMSO in my assay. Can I use PiColorLock Gold?

    A: Yes, the reagent is designed for drug screening work and other situations that require DMSO.

  • Q: I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample

    A: This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.

  • Q: I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?

    A: Yes, only the phosphate will give a signal; pyrophosphate will not.

  • Q: I have phosphate in my enzyme. What can I do?

    A: You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.

  • Q: I have 5% DMSO in my assay. Can I use PiColorLock Gold?

    A: Yes, the reagent is designed for drug screening work and other situations that require DMSO.

  • Q: I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample

    A: This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.

  • Q: I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?

    A: Yes, only the phosphate will give a signal; pyrophosphate will not.

  • Q: I have phosphate in my enzyme. What can I do?

    A: You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.

  • Q: I have 5% DMSO in my assay. Can I use PiColorLock Gold?

    A: Yes, the reagent is designed for drug screening work and other situations that require DMSO.

  • Q: I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample

    A: This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.

  • Q: I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?

    A: Yes, only the phosphate will give a signal; pyrophosphate will not.

  • Q: I have phosphate in my enzyme. What can I do?

    A: You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.

  • Q: I have 5% DMSO in my assay. Can I use PiColorLock Gold?

    A: Yes, the reagent is designed for drug screening work and other situations that require DMSO.

  • Q: I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample

    A: This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.

  • Q: I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?

    A: Yes, only the phosphate will give a signal; pyrophosphate will not.

Showing  1 - 4 of 4 FAQs Showing All
View all FAQs for Antibodies