ATPB Antibody (4.3E8.D10)

Immunocytochemistry/ Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171]

Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATP Synthase beta monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. ATP Synthase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunoprecipitation: ATPB Antibody (4.3E8.D10) [NB600-1171]

Immunoprecipitation: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of rat neuronal/glial cell extract.

Immunoprecipitation: ATPB Antibody (4.3E8.D10) [NB600-1171]

Immunoprecipitation: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta was performed on THP-1 cells. Antigen-antibody complexes were formed by incubating 500ug of THP-1 whole cell lysate (in 500ul volume) with 5ul of an ATP Synthase beta monoclonal antibody overnight at 4C. The immune complexes were captured on 30ul of protein G agarose, washed extensively, and eluted with 6X Laemmli non-reducing sample buffer. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with an ATP Synthase beta monoclonal antibody at a dilution of 1:1000 overnight at 4C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary at a dilution of 1:40,000 for 1 hour at room temperature. Chemiluminescent detection was performed using ECL substrate. Data courtesy of the Innovators Program.