ATR [p Thr1989] Antibody (222) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43564

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 222

Format

Azide and BSA Free
View all ATR Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr1989 of human ATR. The exact sequence is proprietary.

Modification

p Thr1989

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

301 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATR [p Thr1989] Antibody (222) - Azide and BSA Free

Immunoprecipitation: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Analysis of ATR protein from HCT-116 whole cell extracts using 5 ug of ATR antibody or ATR antibody. Western blot analysis was performed using ATR antibody. EasyBlot anti-Mouse IgG was used as a secondary reagent.
ATR [p Thr1989] Antibody (222)

Immunocytochemistry/ Immunofluorescence: ATR [p Thr1989] Antibody (222) [NBP2-43564] -

Immunocytochemistry/ Immunofluorescence: ATR [p Thr1989] Antibody (222) [NBP2-43564] - ATR antibody [GT222] detects ATR protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATR stained by ATR antibody [GT222] (NBP2-43564) diluted at 1:500.
Blue: Fluoroshield with DAPI.
ATR [p Thr1989] Antibody (222)

Western Blot: ATR [p Thr1989] Antibody (222) [NBP2-43564] -

Western Blot: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [GT222] (NBP2-43564) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
ATR [p Thr1989] Antibody (222)

Immunocytochemistry/ Immunofluorescence: ATR [p Thr1989] Antibody (222) [NBP2-43564] -

Immunocytochemistry/ Immunofluorescence: ATR [p Thr1989] Antibody (222) [NBP2-43564] - ATR antibody [GT222] detects ATR protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATR stained by ATR antibody [GT222] (NBP2-43564) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000.
ATR [p Thr1989] Antibody (222) - Azide and BSA Free

Western Blot: ATR [p Thr1989] Antibody (222) [NBP2-43564] -

Untreated (-) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [GT222] (NBP2-43564) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Applications for ATR [p Thr1989] Antibody (222) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATR

Ataxia Telangiectasia Mutated (ATM) and Rad3- related (ATR) protein is a member of the phosphatidyl-inositol 3-kinase (PI 3-kinase) like family of protein kinases. ATR has a predicted molecular mass of approximately 301 kDa, and it has been shown to be involved in cellular responses to DNA damage. Specifically, it plays a role in checkpoint activation in response to either the presence of stalled replication forks, or by the presence of lesions caused by UV light. Inactivation of ATR is an embryonic lethal event in mice.

Long Name

Ataxia Telangiectasia and Rad 3-related

Alternate Names

FRP1, MEC1, SCKL1

Gene Symbol

ATR

Additional ATR Products

Product Documents for ATR [p Thr1989] Antibody (222) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ATR [p Thr1989] Antibody (222) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

p53 Pathway

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Protocols

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FAQs

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