AU1 Epitope Tag Antibody - BSA Free
Novus Biologicals | Catalog # NB600-453
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Cited:
Human, Epitope Tag
Applications
Validated:
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Rabbits were immunized with AU1 (DTYRYI) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AU1 Epitope Tag Antibody - BSA Free
Western Blot: AU1 Epitope Tag AntibodyBSA Free [NB600-453]
Western Blot: AU1 Epitope Tag Antibody [NB600-453] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/mL (1:25,000).Western Blot: AU1 Epitope Tag AntibodyBSA Free [NB600-453]
Western Blot: AU1 Epitope Tag Antibody [NB600-453] - analysis of overexpressed AU1-tagged recombinant proteins in S2 cell extract. Expected lanes are marked with asterisk (*). WB image submitted by a verified customer review.Applications for AU1 Epitope Tag Antibody - BSA Free
Application
Recommended Usage
ELISA
1:1000 - 1:3000
Immunocytochemistry/ Immunofluorescence
1:100 - 1:400
Immunoprecipitation
1 - 4 ug/mg lysate
Western Blot
1:1000 - 1:3000
Application Notes
AU1 Epitope Tag antibody validated for WB, IF from a verified customer reviews.
Reviewed Applications
Read 2 reviews rated 4 using NB600-453 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: AU1 Epitope Tag
Alternate Names
AU1 epitope tag, DTYRYI epitope tag, DTYRYI tag
Additional AU1 Epitope Tag Products
Product Documents for AU1 Epitope Tag Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AU1 Epitope Tag Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for AU1 Epitope Tag Antibody - BSA Free
Customer Reviews for AU1 Epitope Tag Antibody - BSA Free (2)
4 out of 5
2 Customer Ratings
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Application: ImmunofluorescenceSample Tested: Expressed in S2 or WR-Dv-1 cell lineSpecies: OtherVerified Customer | Posted 01/13/2015
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Application: Western BlotSample Tested: S2 lysate (with overexpressed recombinant protein)Species: OtherVerified Customer | Posted 01/13/2015Overexpressed AU1-tagged recombinant proteins in S2 cell extract. Expected lanes are marked with asterisk (*).
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
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- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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