Aurora A Antibody (1F8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80587

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat, Primate

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1F8

Format

Azide and BSA Free
View all Aurora A Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Aurora A expressed in E. coli. [Uniprot: O14965]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aurora A Antibody (1F8) - Azide and BSA Free

Western Blot: Aurora A Antibody (1F8)Azide and BSA Free [NBP2-80587]

Western Blot: Aurora A Antibody (1F8)Azide and BSA Free [NBP2-80587]

Western Blot: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587]

Immunocytochemistry/ Immunofluorescence: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587]

Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587] - Analysis of Hela cells using Aurora A mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Image from the standard format of this antibody.
Flow Cytometry: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587]

Flow Cytometry: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587]

Flow Cytometry: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587] - Analysis of K562 cells using Aurora A mouse mAb (green) and negative control (purple). Image from the standard format of this antibody.
ELISA: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587]

ELISA: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587]

ELISA: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng). Image from the standard format of this antibody.

Applications for Aurora A Antibody (1F8) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aurora A

Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.

Alternate Names

AIK, ARK1, AURA, AURKA, BTAK, STK15, STK6, STK7

Gene Symbol

AURKA

Additional Aurora A Products

Product Documents for Aurora A Antibody (1F8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Aurora A Antibody (1F8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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