Aurora A [p Thr288] Antibody

Novus Biologicals | Catalog # NB100-2371

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Porcine

Cited:

Human, Mouse, Porcine, Bovine, Ovine

Applications

Validated:

Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Western Blot (Negative)

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region surrounding residue 288 when Threonine 288 is phosphorylated of human Aurora A Kinase using the numbering given entry NP_003591.2 (GeneID 6790).

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 24983972). Mouse reactivity reported in scientific literature (PMID: 26174602).

Modification

p Thr288

Marker

Mitosis Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aurora A [p Thr288] Antibody

Immunocytochemistry/ Immunofluorescence: Aurora A [p Thr288] Antibody [NB100-2371]

Immunocytochemistry/ Immunofluorescence: Aurora A [p Thr288] Antibody [NB100-2371]

Immunocytochemistry/Immunofluorescence: Aurora A [p Thr288] Antibody [NB100-2371] - Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho-Aurora A (T288) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.
Immunocytochemistry/ Immunofluorescence: Aurora A [p Thr288] Antibody [NB100-2371]

Immunocytochemistry/ Immunofluorescence: Aurora A [p Thr288] Antibody [NB100-2371]

Immunocytochemistry/Immunofluorescence: Aurora A [p Thr288] Antibody [NB100-2371] - NBF-fixed asynchronous HeLa cells. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-Aurora A. Antibody: Affinity purified rabbit anti- Phospho-Aurora A (T288) (NB100-2371) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated (NB7159) used at a dilution of 1:100.

Applications for Aurora A [p Thr288] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:10-1:500
Application Notes
Formaldehyde-Fixed Cells: permeabilization with Triton-X 100 is recommended. Likely to work with frozen sections. IHC was reported in scientific literature (PMID: 23649868). Antibody has been tested for Western Blots on numerous occasions and has not been successfully used.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.25 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Aurora A

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.

Alternate Names

AIK, ARK1, AURA, AURKA, BTAK, STK15, STK6, STK7

Entrez Gene IDs

6790 (Human)

Gene Symbol

AURKA

UniProt

NP_003591.2 (Human)

Additional Aurora A Products

Product Documents for Aurora A [p Thr288] Antibody

Certificate of Analysis

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Product Specific Notices for Aurora A [p Thr288] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Aurora A [p Thr288] Antibody

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Protocols

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