aurora A-binding protein Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-81152
![Western Blot: aurora A-binding protein Antibody [NBP1-81152]](https://resources.rndsystems.com/images/products/aurora-A-binding-protein-Antibody-Western-Blot-NBP1-81152-img0007.jpg "Western Blot: aurora A-binding protein Antibody [NBP1-81152]")
Loading...
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Virus
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CGVWLDAAALKRRKVQTHLIKPGTKMLTLLPGERKANIYFTQRRAPSTGIHQRSIASFFTLQPGKTNGSDQTSVSSHTESQIN
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for aurora A-binding protein Antibody - BSA Free
Western Blot: aurora A-binding protein Antibody [NBP1-81152]
Western Blot: aurora A-binding protein Antibody [NBP1-81152] - Analysis in control (vector only transfected HEK293T lysate) and AUNIP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: aurora A-binding protein Antibody [NBP1-81152]](https://resources.rndsystems.com/images/products/aurora-A-binding-protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81152-img0006.jpg "Immunocytochemistry/ Immunofluorescence: aurora A-binding protein Antibody [NBP1-81152]")
Immunocytochemistry/ Immunofluorescence: aurora A-binding protein Antibody [NBP1-81152]
Immunocytochemistry/Immunofluorescence: aurora A-binding protein Antibody [NBP1-81152] - Staining of human cell line U-251 MG shows localization to centrosome. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: aurora A-binding protein Antibody [NBP1-81152]](https://resources.rndsystems.com/images/products/aurora-A-binding-protein-Antibody-Immunohistochemistry-Paraffin-NBP1-81152-img0001.jpg "Immunohistochemistry-Paraffin: aurora A-binding protein Antibody [NBP1-81152]")
Immunohistochemistry-Paraffin: aurora A-binding protein Antibody [NBP1-81152]
Immunohistochemistry-Paraffin: aurora A-binding protein Antibody [NBP1-81152] - Staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.
Chromatin Immunoprecipitation ChIP: aurora A-binding protein Antibody - BSA Free
ChIP-Exo-Seq composite graph for Anti-aurora A-binding protein tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.Applications for aurora A-binding protein Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Knockdown validation (PMID: 31964734).
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: aurora A-binding protein
Alternate Names
AIBP, aurora A-binding protein, chromosome 1 open reading frame 135, FLJ14264, MGC2603
Entrez Gene IDs
79000 (Human)
Gene Symbol
AUNIP
Additional aurora A-binding protein Products
Product Documents for aurora A-binding protein Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for aurora A-binding protein Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for aurora A-binding protein Antibody - BSA Free
Powered by Bioz
Customer Reviews for aurora A-binding protein Antibody - BSA Free
There are currently no reviews for this product. Be the first to review aurora A-binding protein Antibody - BSA Free and earn rewards!
Have you used aurora A-binding protein Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...