B7-1/CD80 Antibody (16-10A1) [PE/Cy5]

Name: B7-1/CD80 Antibody (16-10A1) [PE/Cy5]
Brand: Novus Biologicals
SKU: NBP1-43086
Price: 519 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-43086

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Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Feline

Applications

Flow Cytometry

Label

PE/Cy5 (Excitation = 480;565;650 nm, Emission = 670 nm)

Antibody Source

Monoclonal Armenian Hamster IgG Clone # 16-10A1

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Product Specifications

Immunogen

The immunogen for this antibody was CD80.

Reactivity Notes

Feline reactivity reported from a verified customer review.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Scientific Data Images for B7-1/CD80 Antibody (16-10A1) [PE/Cy5]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [PE/Cy5] [NBP1-43086] - Staining of 3-day LPS activated C57Bl/6 splenocytes with 0.03 ug of PE-Cy5 Armenian Hamster IgG Isotype Control (open histogram) or 0.03 ug of Anti-Mouse CD80 (B7-1) PE-Cy5 (filled histogram). Total cells were used for analysis.

Applications for B7-1/CD80 Antibody (16-10A1) [PE/Cy5]

Application

Recommended Usage

Flow Cytometry

1:10-1:1000

Application Notes

The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2) with 0.1% gelatin

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: B7-1/CD80

The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

Alternate Names

B71, CD80

Gene Symbol

CD80

Additional B7-1/CD80 Products

Product Documents for B7-1/CD80 Antibody (16-10A1) [PE/Cy5]

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Certificate of Analysis

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Product Specific Notices for B7-1/CD80 Antibody (16-10A1) [PE/Cy5]

Cy is a registered trademark of Global Life Sciences Solutions Germany GmbH.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.