B7-1/CD80 Antibody (16-10A1) - BSA Free

Novus Biologicals | Catalog # NBP1-43385

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Feline

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # 16-10A1

Format

BSA Free
View all B7-1/CD80 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD80.

Reactivity Notes

Feline reactivity reported from a verified customer review.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Description

Novus Biologicals Armenian Hamster B7-1/CD80 Antibody (16-10A1) - BSA Free (NBP1-43385) is a monoclonal antibody validated for use in IHC, Flow and IP. Anti-B7-1/CD80 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for B7-1/CD80 Antibody (16-10A1) - BSA Free

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the PE/Cy5 conjugate of NBP1-43385. Staining of 3-day LPS activated C57Bl/6 splenocytes with 0.03 ug of PE-Cy5 Armenian Hamster IgG Isotype Control (open histogram) or 0.03 ug of Anti-Mouse CD80 (B7-1) PE-Cy5 (filled histogram). Total cells
Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD80 (B7-1) Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC. Total viable cells were used for analysis.
Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the Biotin conjugate of NBP1-43385. Staining of 3-day LPS-stimulated C57BL/6 splenocytes with 0.06 ug of Armenian Hamster IgG Isotype Control Biotin (open histogram) or 0.06 ug of Anti-Mouse CD80 (B7-1) Biotin (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.
Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the FITC conjugate of NBP1-43385. Staining of unstimulated (left) or 3-day LPS-stimulated (right) C57BL/6 splenocytes with 0.125 ug of Anti-Mouse CD80 (B7-1) FITC. Total viable cells were used for analysis. Markers were set based on the autofluorescence sample.
Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the PE conjugate of NBP1-43385. See Notes
B7-1/CD80 Antibody (16-10A1) - BSA Free

Flow Cytometry: Armenian Hamster Monoclonal B7-1/CD80 Antibody (16-10A1) [NBP1-43385]

Flow Cytometry: Armenian Hamster Monoclonal B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Alexa Fluor® 488 conjugated antibody (Catalog # NBP1-43385AF488) was tested via flow cytometry using magnetic beads coated with feline protein. Histogram overlay showing fluorescence results obtained via flow cytometry. Red line (unstained beads) and green line (beads coated with CD86 synthetic protein) served as negative controls. Blue line: positive signal obtained with CD80-coated beads. Image from a verified customer review.

Applications for B7-1/CD80 Antibody (16-10A1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: B7-1/CD80

The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

Alternate Names

B71, CD80

Gene Symbol

CD80

Additional B7-1/CD80 Products

Product Documents for B7-1/CD80 Antibody (16-10A1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for B7-1/CD80 Antibody (16-10A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for B7-1/CD80 Antibody (16-10A1) - BSA Free

Customer Reviews for B7-1/CD80 Antibody (16-10A1) - BSA Free

There are currently no reviews for this product. Be the first to review B7-1/CD80 Antibody (16-10A1) - BSA Free and earn rewards!

Have you used B7-1/CD80 Antibody (16-10A1) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for B7-1/CD80 Antibody (16-10A1) - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

    • A: Please note that CD80 is a transmembrane protein with extracellular, helical and cytoplasmic domains. Accordingly, in immunostaining assays, you can expect staining at and around the cellular membranes.
Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies