B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-09018

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # HK5.3

Format

Azide and BSA Free
View all B7-H2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunizing SD rat with mouse B7h-transfected L cells and fusing immune splenocytes with P3U1 myeloma cells and screened for binding to mouse B7h-transfected NRK cells.

Specificity

This antibody specifically binds to mouse ICOSL and competes with binding of ICOS.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free [NBP3-09018]

Immunocytochemistry/ Immunofluorescence: B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free [NBP3-09018]

Immunocytochemistry/Immunofluorescence: B7-H2/ICOSLG Antibody (HK5.3) - Chimeric [NBP3-09018] - Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of HK5.3 (NBP3-09018) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing weak membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09018, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.
ELISA: B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free [NBP3-09018]

ELISA: B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free [NBP3-09018]

ELISA: B7-H2/ICOSLG Antibody (HK5.3) - Chimeric [NBP3-09018] - ELISA Plate coated with B7-H2/ICOSLG-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP3-09018. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody was used.

Applications for B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

This antibody has been used to detect murine ICOSL by flow cytometry. It is able to block ICOSL interacting with ICOSL and inhibit T-cell proliferation in a collagen type II (CII)-induced arthritis model.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B7-H2

B7-RP1 is a 40 kD protein, also known as ICOS ligand, B7-H2, and B7h. It has recently been clustered as CD275. It is a member of the immunoglobulin receptor superfamily expressed on splenic B cells, dendritic cells and macrophages. The expression of this antigen is increased by TNF- alpha stimulation and is highly upregulated by local inflammation. B7RP1 binds to ICOS and co-stimulates T cell and B cell responses. Two isoforms of this protein have been reported to occur as a result of alternative splicing. These isoforms show differential tissue distribution.

Long Name

B7 Homolog 2

Alternate Names

B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG

Gene Symbol

ICOSLG

Additional B7-H2 Products

Product Documents for B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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