B7-H6 Antibody (17B1.3) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-09050

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 17B1.3

Format

Azide and BSA Free
View all B7-H6 Primary Antibodies »

Product Specifications

Immunogen

Immunised mice with extracellular domain of human B7-H6 and selected on the basis of specific reactivity by P815.B7-H6, but not with P815.B7-H1.

Specificity

mAb 17B1.3 specifically recognises B7-H6. 17B1.3 reacted with human tumour cell lines constitutively expressing B7-H6 such as HeLa cells. Fab 17B1.3 does not interfere with the binding of B7-H6 to NKp30, but nevertheless blocks NK cell activation.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for B7-H6 Antibody (17B1.3) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: B7-H6 Antibody (17B1.3) - Chimeric - Azide and BSA Free [NBP3-09050]

Immunocytochemistry/ Immunofluorescence: B7-H6 Antibody (17B1.3) - Chimeric - Azide and BSA Free [NBP3-09050]

Immunocytochemistry/Immunofluorescence: B7-H6 Antibody (17B1.3) - Chimeric [NBP3-09050] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 17B1.3 (NBP3-09050) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09050, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
B7-H6 Antibody (17B1.3) - Chimeric - Azide and BSA Free

Flow Cytometry: B7-H6 Antibody (17B1.3) - Chimeric - Azide and BSA Free [NBP3-09050] -

Flow Cytometry: B7-H6 Antibody (17B1.3) - Chimeric - Azide and BSA Free [NBP3-09050] - Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-09050 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications for B7-H6 Antibody (17B1.3) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

B7-H6 is not detected in normal human tissues at steady state but is expressed on tumour cells, so tumour cells can be detected by NK cells. This article describes the study of the mechanisms that govern the induction of B7-H6 on primary cells as well as its consequences in vitro and in vivo.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B7-H6

B7-H6 is a transmembrane immune regulatory protein that binds to NKp30 expressed on NK cells. Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells. The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30-dependent cell lysis.

Long Name

B7 Homolog 6

Alternate Names

B7H6, DKFZp686I21167, DKFZp686O24166, NCR3LG1

Gene Symbol

NCR3LG1

Additional B7-H6 Products

Product Documents for B7-H6 Antibody (17B1.3) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for B7-H6 Antibody (17B1.3) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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