Bafilomycin A1

Catalog # Availability Size / Price Qty
Bafilomycin A1 | CAS No. 88899-55-2 | H+-ATPase Inhibitors
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Description: H+-ATPase (vacuolar) inhibitor

Chemical Name: (3Z,5E,7R,8S,9S,11E,13E,15S,16R)-8-Hydroxy-16-[(1S,2R,3S)-2-hydroxy-1-methyl-3-[(2R,4R,5S,6R)-tetrahydro-2,4-dihydroxy-5-methyl-6-(1-methylethyl)-2H-pyran-2-yl]butyl]-3,15-dimethoxy-5,7,9,11-tetramethyloxacyclohexadeca-3,5,11,13-tetraen-2-one

Purity: ≥95%

Product Details
Citations (52)

Biological Activity

Bafilomycin A1 is a highly potent, selective inhibitor of vacuolar H+-ATPases (IC50 = 0.6 - 1.5 nM in bovine chromaffin granules). Selective for v-ATPase over other ATP hydrolyzing enzymes such as F-ATPases and the H+/K+-ATPase (P-ATPase). Thought to inhibit autophagy either by blocking autophagosome-lysosome fusion (in H4IIE cells), or by blocking lysosomal degradation. Bafilomycin A1 accelerates wound healing in db/db mice. Low dose Bafilomycin A1 attenuates patient-derived CD34+CD19+ leukemia stem cells (LSC) and inhibits LSC proliferation in an animal model. Bafilomycin A1 impairs Zika virus infection in vitro.

Technical Data

Soluble to 5 mg/ml in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Citations for Bafilomycin A1

The citations listed below are publications that use Tocris products. Selected citations for Bafilomycin A1 include:

52 Citations: Showing 1 - 10

  1. TGF-β-induced DACT1 biomolecular condensates repress Wnt signalling to promote bone metastasis.
    Authors: Esposito Et al.
    Nat.Cell.Biol.  2021;23:257
  2. Age-dependent accumulation of oligomeric SNCA/α-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA).
    Authors: Ho Et al.
    Autophagy  2019;:1
  3. The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.
    Authors: Huang Et al.
    Autophagy  2019;:1
  4. Autophagic cell death restricts chromosomal instability during replicative crisis.
    Authors: Nassour Et al.
    Nature  2019;565:659
  5. Lethal Poisoning of Cancer Cells by Respiratory Chain Inhibition plus Dimethyl α-Ketoglutarate.
    Authors: Sica Et al.
    Cell Rep  2019;27:820
  6. Absence of Cytosolic 2-Cys Prx Subtypes I and II Exacerbates TNF-α-Induced Apoptosis via Different Routes.
    Authors: Lee Et al.
    Cell Rep  2019;26:2194
  7. Recruitment of LC3 to damaged Golgi apparatus.
    Authors: Gomes-da-Silva Et al.
    Cell Death Differ  2019;26:1467
  8. Anti-MET VHH Pool Overcomes MET-Targeted Cancer Therapeutic Resistance.
    Authors: Su Et al.
    Mol Cancer Ther  2019;18:100
  9. Junín virus induces autophagy in human A549 cells.
    Authors: Vidakovics Et al.
    PLoS One  2019;14:e0218730
  10. Melatonin: The smart molecule that differentially modulates autophagy in tumor and normal placental cells.
    Authors: Sagrillo-Fagundes Et al.
    PLoS One  2019;14:e0202458
  11. Oligodendrocytes express synaptic proteins that modulate myelin sheath formation.
    Authors: Hughes and Appel
    Nat Commun  2019;10:4125
  12. IL-34 and CSF-1 display an equivalent macrophage differentiation ability but a different polarization potential.
    Authors: Boulakirba Et al.
    Sci Rep  2018;8:256
  13. A role for proteolytic regulation of δ-catenin in remodeling a subpopulation of dendritic spines in the rodent brain.
    Authors: Yuan Et al.
    J Biol Chem  2018;293:11625
  14. Dynamin inhibitors block activation of mTORC1 by amino acids independently of dynamin.
    Authors: Persaud Et al.
    J Cell Sci  2018;131
  15. Lack of evidence for involvement of TonEBP and hyperosmotic stimulus in induction of autophagy in the nucleus pulposus.
    Authors: Liu Et al.
    Sci Rep  2017;7:4543
  16. Ferroquine, the next generation antimalarial drug, has antitumor activity.
    Authors: Kondratskyi Et al.
    Sci Rep  2017;7:15896
  17. Proteome-wide Map of Targets of T790M-EGFR-Directed Covalent Inhibitors.
    Authors: Niessen Et al.
    Cell Chem Biol  2017;24:1388
  18. Pharmacological modulators of autophagy activate a parallel noncanonical pathway driving unconventional LC3 lipidation.
    Authors: Jacquin Et al.
    Autophagy  2017;13:854
  19. Methyl-β-cyclodextrin restores impaired autophagy flux in Niemann-Pick C1-deficient cells through activation of AMPK.
    Authors: Dai Et al.
    Autophagy  2017;13:1435
  20. Cannabinoids synergize with carfilzomib, reducing multiple myeloma cells viability and migration.
    Authors: Nabissi Et al.
    Oncotarget  2016;7:77543
  21. Down regulated lncRNA MEG3 eliminates mycobacteria in macrophages via autophagy.
    Authors: Pawar Et al.
    Radiat Oncol  2016;6:19416
  22. Functional CRISPR screening identifies the ufmylation pathway as a regulator of SQSTM1/p62.
    Authors: DeJesus Et al.
    Elife  2016;5
  23. Hypoxia promotes noncanonical autophagy in nucleus pulposus cells independent of MTOR and HIF1A signaling.
    Authors: Choi Et al.
    Autophagy  2016;12:1631
  24. Trehalose upregulates progranulin expression in human and mouse models of GRN haploinsufficiency: a novel therapeutic lead to treat frontotemporal dementia.
    Authors: Holler Et al.
    Mol Neurodegener  2016;11:46
  25. Lysosomal two-pore channel subtype 2 (TPC2) regulates skeletal muscle autophagic signaling.
    Authors: Lin Et al.
    Sci Rep  2015;290:3377
  26. Calcium release through P2X4 activates calmodulin to promote endolysosomal membrane fusion.
    Authors: Cao Et al.
    Int J Mol Sci  2015;209:879
  27. Docosahexaenoic Acid Induces Cell Death in Human Non-Small Cell Lung Cancer Cells by Repressing mTOR via AMPK Activation and PI3K/Akt Inhibition.
    Authors: Kim Et al.
    Biomed Res Int  2015;2015:239764
  28. Palmitoylethanolamide inhibits glutamate release in rat cerebrocortical nerve terminals.
    Authors: Lin Et al.
    J Clin Invest  2015;16:5555
  29. Cytosolic HMGB1 controls the cellular autophagy/apoptosis checkpoint during inflammation.
    Authors: Zhu Et al.
    J Biol Chem  2015;125:1098
  30. Cardiolipin remodeling by TAZ/tafazzin is selectively required for the initiation of mitophagy.
    Authors: Hsu Et al.
    Autophagy  2015;11:643
  31. The PRKAA1/AMPKα1 pathway triggers autophagy during CSF1-induced human monocyte differentiation and is a potential target in CMML.
    Authors: Obba Et al.
    J Cell Biol  2015;11:1114
  32. Release of monocyte migration signals by breast cancer cell lines after ablative and fractionated γ-irradiation.
    Authors: Hennel Et al.
    Mol Biol Cell  2014;9:85
  33. Identification of ML-9 as a lysosomotropic agent targeting autophagy and cell death.
    Authors: Kondratskyi Et al.
    Cell Death Dis  2014;5:e1193
  34. Phosphorylation of synapsin I by cyclin-dependent kinase-5 sets the ratio between the resting and recycling pools of synaptic vesicles at hippocampal synapses.
    Authors: Verstegen Et al.
    J Neurosci  2014;34:7266
  35. The anticancer agent di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT) overcomes prosurvival autophagy by two mechanisms: persistent induction of autophagosome synthesis and impairment of lysosomal integrity.
    Authors: Gutierrez Et al.
    J Biol Chem  2014;289:33568
  36. Chloroquine promotes IL-17 production by CD4+ T cells via p38-dependent IL-23 release by monocyte-derived Langerhans-like cells.
    Authors: Said Et al.
    J Immunol  2014;193:6135
  37. Polarized granzyme release is required for antigen-driven transendothelial migration of human effector memory CD4 T cells.
    Authors: Manes and Pober
    J Immunol  2014;193:5809
  38. Cell-intrinsic lysosomal lipolysis is essential for alternative activation of macrophages.
    Authors: Huang Et al.
    Nat Immunol  2014;15:846
  39. Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.
    Authors: McAlpine Et al.
    Autophagy  2013;9:361
  40. Characteristics and requirements of basal autophagy in HEK 293 cells.
    Authors: Musiwaro Et al.
    Autophagy  2013;9:1407
  41. Pulsed electromagnetic fields increased the anti-inflammatory effect of A2A and A3 adenosine receptors in human T/C-28a2 chondrocytes and hFOB 1.19 osteoblasts.
    Authors: Vincenzi Et al.
    PLoS One  2013;8:e65561
  42. Autophagy-mediated turnover of dynamin-related protein 1.
    Authors: Purnell and Fox
    BMC Neurosci  2013;14:86
  43. The anti-apoptotic Bcl-B protein inhibits BECN1-dependent autophagic cell death.
    Authors: Robert Et al.
    Autophagy  2012;8:637
  44. Immunohistochemical detection of cytoplasmic LC3 puncta in human cancer specimens.
    Authors: Ladoire Et al.
    Autophagy  2012;8:1175
  45. West Nile virus growth is independent of autophagy activation.
    Authors: Beatman Et al.
    Virology  2012;433:262
  46. Negative regulation of signaling by a soluble form of toll-like receptor 9.
    Authors: Chockalingam Et al.
    Eur J Immunol  2011;41:2176
  47. Post-intoxication inhibition of botulinum neurotoxin serotype A within neurons by small-molecule, non-peptidic inhibitors.
    Authors: Ruthel Et al.
    Toxins (Basel)  2011;3:207
  48. Relieving autophagy and 4EBP1 from rapamycin resistance.
    Authors: Nyfeler Et al.
    Mol Cell Biol  2011;31:2867
  49. Notch post-translationally regulates β-catenin protein in stem and progenitor cells.
    Authors: Kwon Et al.
    Nat Cell Biol  2011;13:1244
  50. Beta1 integrin-dependent engulfment of Yersinia enterocolitica by macrophages is coupled to the activation of autophagy and suppressed by type III protein secretion.
    Authors: Deuretzbacher Et al.
    J Immunol  2009;183:5847
  51. Extrasynaptic release of GABA by retinal DArgic neurons.
    Authors: Hirasawa Et al.
    J Neurophysiol  2009;102:146
  52. Genome-wide siRNA-based functional genomics of pigmentation identifies novel genes and pathways that impact melanogenesis in human cells.
    Authors: Ganesan Et al.
    PLoS Genet  2008;4:e1000298


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