Bag-1 Antibody (1E4B5) - BSA Free

Novus Biologicals | Catalog # NBP2-61698

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1E4B5

Format

BSA Free
View all Bag-1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Bag-1 (AA: 219-346) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bag-1 Antibody (1E4B5) - BSA Free

Western Blot: Bag-1 Antibody (1E4B5)BSA Free [NBP2-61698]

Western Blot: Bag-1 Antibody (1E4B5)BSA Free [NBP2-61698]

Western Blot: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis using BAG1 mAb against human BAG1 (AA: 219-346) recombinant protein. (Expected MW is 40.6 kDa)
Immunocytochemistry/ Immunofluorescence: Bag-1 Antibody (1E4B5) - BSA Free [NBP2-61698]

Immunocytochemistry/ Immunofluorescence: Bag-1 Antibody (1E4B5) - BSA Free [NBP2-61698]

Immunocytochemistry/Immunofluorescence: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis of Hela cells using BAG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Flow Cytometry: Bag-1 Antibody (1E4B5) - BSA Free [NBP2-61698]

Flow Cytometry: Bag-1 Antibody (1E4B5) - BSA Free [NBP2-61698]

Flow Cytometry: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis of Hela cells using BAG1 mouse mAb (green) and negative control (red).
Western Blot: Bag-1 Antibody (1E4B5)BSA Free [NBP2-61698]

Western Blot: Bag-1 Antibody (1E4B5)BSA Free [NBP2-61698]

Western Blot: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis using BAG1 mAb against HEK293 (1) and BAG1 (AA: 219-346)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: Bag-1 Antibody (1E4B5) - BSA Free [NBP2-61698]

ELISA: Bag-1 Antibody (1E4B5) - BSA Free [NBP2-61698]

ELISA: Bag-1 Antibody (1E4B5) [NBP2-61698] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for Bag-1 Antibody (1E4B5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:500-1:2000

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bag-1

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X.

Long Name

Bcl-2-associated Anthanogene-1

Alternate Names

Bag1

Gene Symbol

BAG1

Additional Bag-1 Products

Product Documents for Bag-1 Antibody (1E4B5) - BSA Free

Certificate of Analysis

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Product Specific Notices for Bag-1 Antibody (1E4B5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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Protocols

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FAQs

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