Bag-1 Antibody (RM310)
Novus Biologicals | Catalog # NBP2-77409
Recombinant Monoclonal Antibody
![Western Blot: Bag-1 Antibody (RM310) [NBP2-77409]](https://resources.rndsystems.com/images/products/Bag-1-Antibody-RM310-Western-Blot-NBP2-77409-img0004.jpg "Western Blot: Bag-1 Antibody (RM310) [NBP2-77409]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM310 expressed in HEK293
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Product Specifications
Immunogen
A peptide corresponding to residues near the N-terminus of human Bag-1
Specificity
This antibody reacts to human Bag-1L. It does not cross react to Bag-1M or Bag-1S.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Bag-1 Antibody (RM310)
Western Blot: Bag-1 Antibody (RM310) [NBP2-77409]
Western Blot: Bag-1 Antibody (RM310) [NBP2-77409] - Western Blot of HeLa cell lysate, using NBP2-77409.![Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409]](https://resources.rndsystems.com/images/products/Bag-1-Antibody-RM310-Immunohistochemistry-NBP2-77409-img0003.jpg "Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409]")
Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409]
Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409] - Immunohistochemical staining of formalin fixed and paraffin embedded BAG-1L overexpressing LNCaP cell section using NBP2-77409.![Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409]](https://resources.rndsystems.com/images/products/Bag-1-Antibody-RM310-Immunohistochemistry-NBP2-77409-img0001.jpg "Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409]")
Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409]
Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409] - Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1 cell section using NBP2-77409.![Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409]](https://resources.rndsystems.com/images/products/Bag-1-Antibody-RM310-Immunohistochemistry-NBP2-77409-img0002.jpg "Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409]")
Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409]
Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409] - Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1 cell section using NBP2-77409.Applications for Bag-1 Antibody (RM310)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Immunohistochemistry
1:500 - 1:2000
Western Blot
1:1000 - 1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Bag-1
Long Name
Bcl-2-associated Anthanogene-1
Alternate Names
Bag1
Gene Symbol
BAG1
Additional Bag-1 Products
Product Documents for Bag-1 Antibody (RM310)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Bag-1 Antibody (RM310)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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