BAG3 Antibody (8P3W7)

Novus Biologicals | Catalog # NBP3-16469

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8P3W7 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human BAG3 (O95817). NDPRVPSEGPKETPSSANGPSREGSRLPPAREGHPVYPQLRPGYIPIPVLHEGAENRQVHPFHVYPQPGMQRFRTEAAAAAPQRSQSPLRGMPETTQPDKQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BAG3 Antibody (8P3W7)

Western Blot: BAG3 Antibody (8P3W7) [NBP3-16469]

Western Blot: BAG3 Antibody (8P3W7) [NBP3-16469]

Western Blot: BAG3 Antibody (8P3W7) [NBP3-16469] - Western blot analysis of extracts of various cell lines, using BAG3 Rabbit mAb (NBP3-16469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: BAG3 Antibody (8P3W7) [NBP3-16469]

Immunohistochemistry-Paraffin: BAG3 Antibody (8P3W7) [NBP3-16469]

Immunohistochemistry-Paraffin: BAG3 Antibody (8P3W7) [NBP3-16469] - Immunohistochemistry of paraffin-embedded mouse kidney using BAG3 Rabbit mAb (NBP3-16469) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for BAG3 Antibody (8P3W7)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BAG3

Alternate Names

BAG family molecular chaperone regulator 3, BAG-3, BAG-family molecular chaperone regulator-3, Bcl-2-associated athanogene 3, BCL2-associated athanogene 3, BCL2-binding athanogene 3, Bcl-2-binding protein Bis, BIS, CAIR-1, Docking protein CAIR-1, MGC104307

Gene Symbol

BAG3

Additional BAG3 Products

Product Documents for BAG3 Antibody (8P3W7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for BAG3 Antibody (8P3W7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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